≡

Active Lysyl Oxidase (LOX)

Protein-Lysine 6-Oxidase

ACTIVITY TEST

Lysyl oxidase (LOX) also known as protein-lysine 6-oxidase is an extracellular copper-dependent enzyme that catalyzes formation of aldehydes from lysine residues in collagen and elastin precursors. Its catalytic activity depends upon both its copper cofactor and a unique carbonyl cofactor and has been shown to extend to a variety of basic globular proteins, including histone H1. LOX plays a major role in connective tissue development and may also be important in neurological function. Lysyl oxidase has also proven crucial to the development of the respiratory system and the skin, the commitment step of adipocyte, and the formation of pluripotent stem cells during development. Its absence may lead to defects in the transforming growth factor beta superfamily of proteins, which control cell growth and differentiation. Besides, Elastin (ELN) has been identified as an interactor of LOX, thus a binding ELISA assay was conducted to detect the interaction of recombinant human LOX and recombinant human ELN. Briefly, LOX were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to ELN-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-LOX pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of LOX and ELN was shown in Figure 1, and this effect was in a dose dependent manner.

USAGE

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
American Journal of Hematology The expression of lysyl-oxidase gene family members in myeloproliferative neoplasms. PubMed: 23494965
Current Eye Research High Glucose Induced Differential Expression of Lysyl Oxidase and Its Isoform in ARPE-19 Cells Pubmed: 22966818
European Journal of Surgical Oncology Evaluation of serum lysyl oxidase as a blood test for colorectal cancer Pubmed: 24246612
Vaccine Copia autorizada por CDR Ingentaconnect: Source
Arthritis & Rheumatology Lysyl oxidase is a potential biomarker of fibrosis in systemic sclerosis Pubmed: 24574233
Eur J Surg Oncol.? Evaluation of serum lysyl oxidase as a blood test for colorectal cancer Pubmed:Pmc4237001
BMC Cancer. Ionizing radiation induces tumor cell lysyl oxidase secretion Pubmed:Pmc4223762
DNA Cell Biol. Lysyl Oxidase Genetic Variants Affect Gene Expression in Cervical Cancer Pubmed:24945327
scandinavian journal of immunology Increased Serum Levels of the IL-33 Neutralizing sST2 in Limited Cutaneous Systemic Sclerosis PubMed: 26095613
Curr Eye Res Correlation of Aqueous Humor Lysyl Oxidase Activity with TGF-ß Levels and LOXL1 Genotype in Pseudoexfoliation Pubmed:27116380
journal of bone and mineral metabolism LOX Fails to Substitute for RANKL in Osteoclastogenesis. pubmed:27606829
38 Blocking Surgically Induced Lysyl Oxidase Activity Reduces the Risk of Lung Metastases. pubmed:28445728
Molecular Medicine Reports Lysyl oxidase is involved in synovial hyperplasia and angiogenesis in rats with collagen‑induced arthritis pubmed:28901438
Revista Española de Cardiología (English Edition) A Global Assessment of Circulating Prolysyl Oxidase in Nonischemic Patients With Garden-variety Heart Failure With Preserved Ejection Fraction Pubmed:29807761
Molecular Medicine Reports Lysyl oxidase inhibition via β-aminoproprionitrile hampers human umbilical vein endothelial cell angiogenesis and migration in vitro Pubmed:29393489
Thyroid Lysyl Oxidase is a key player in BRAF/MAPK pathway-driven thyroid cancer aggressiveness Pubmed: 30398411
Am J Physiol Lung Cell Mol Physiol Lysyl Oxidase Directly Contributes to Extracellular Matrix Production and Fibrosis in Systemic Sclerosis. Pubmed: 33026236
J Am Heart Assoc Collagen Cross©\Linking Is Associated With Cardiac Remodeling in Hypertrophic Obstructive Cardiomyopathy 33356379
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
APC580Hu01 Active Lysyl Oxidase (LOX) Cell culture; Activity Assays.
RPC580Hu01 Recombinant Lysyl Oxidase (LOX) Positive Control; Immunogen; SDS-PAGE; WB.
RPC580Hu02 Recombinant Lysyl Oxidase (LOX) Positive Control; Immunogen; SDS-PAGE; WB.
EPC580Hu61 Eukaryotic Lysyl Oxidase (LOX) Positive Control; Immunogen; SDS-PAGE; WB.
PAC580Hu02 Polyclonal Antibody to Lysyl Oxidase (LOX) WB; IHC; ICC; IP.
PAC580Hu01 Polyclonal Antibody to Lysyl Oxidase (LOX) WB; IHC; ICC; IP.
LAC580Hu81 FITC-Linked Polyclonal Antibody to Lysyl Oxidase (LOX) WB; IHC; ICC; IF.
LAC580Hu71 Biotin-Linked Polyclonal Antibody to Lysyl Oxidase (LOX) WB; IHC; ICC.
MAC580Hu22 Monoclonal Antibody to Lysyl Oxidase (LOX) WB; IHC; ICC; IP.
SEC580Hu ELISA Kit for Lysyl Oxidase (LOX) Enzyme-linked immunosorbent assay for Antigen Detection.
WEC580Hu Wide-range ELISA Kit for Lysyl Oxidase (LOX) Enzyme-linked immunosorbent assay for Antigen Detection.
LMC580Hu Multiplex Assay Kit for Lysyl Oxidase (LOX) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
KSC580Hu01 ELISA Kit DIY Materials for Lysyl Oxidase (LOX) Main materials for "Do It(ELISA Kit) Yourself"