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Active Endonuclease G, Mitochondrial (ENDOG)

ENDO-G

ACTIVITY TEST

Endonuclease G, Mitochondrial (ENDOG) is a conserved nuclease encoded by nuclear DNA and localized in the mitochondrial intermembrane space. It plays dual roles in apoptosis and mitochondrial genome maintenance. During programmed cell death, ENDOG is released into the cytosol, cleaving nuclear and mitochondrial DNA at specific sites to facilitate apoptotic degradation. Structurally, it functions as a homodimer with Mg²⁺-dependent endonuclease activity. Beyond apoptosis, ENDOG participates in mitochondrial DNA replication, repair, and maternal genome processing during embryogenesis. Notably, ENDOG interacts with cytochrome c (CYCS) to synergistically amplify apoptosis. This complex enhances nucleolytic DNA degradation and modulates the mitochondrial permeability transition pore (MPTP), further promoting cell death.Thus a functional ELISA assay was conducted to detect the interaction of recombinant rat ENDOG and recombinant human CYCS. Briefly, ENDOG was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to CYCS-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti-ENDOG pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37℃, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 µL stop solution to the wells and read at 450/630nm immediately. The binding activity of recombinant rat ENDOG and recombinant human CYCS was shown in Figure 1, the EC50 for this effect is 1.01ug/mL.

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Catalog No. Related products for research use of Rattus norvegicus (Rat) Organism species Applications (RESEARCH USE ONLY!)
RPC457Ra01 Recombinant Endonuclease G, Mitochondrial (ENDOG) Positive Control; Immunogen; SDS-PAGE; WB.
APC457Ra01 Active Endonuclease G, Mitochondrial (ENDOG) Cell culture; Activity Assays.
PAC457Ra01 Polyclonal Antibody to Endonuclease G, Mitochondrial (ENDOG) WB; IHC; ICC; IP.
MAC457Ra21 Monoclonal Antibody to Endonuclease G, Mitochondrial (ENDOG) WB; IHC; ICC; IP.
SEC457Ra ELISA Kit for Endonuclease G, Mitochondrial (ENDOG) Enzyme-linked immunosorbent assay for Antigen Detection.
LMC457Ra Multiplex Assay Kit for Endonuclease G, Mitochondrial (ENDOG) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.