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Active Cathelicidin Antimicrobial Peptide (CAMP)

CAP18; FALL39; HSD26; LL37; 18 kDa Cationic Antimicrobial Protein; Antibacterial protein FALL-39; FALL-39 peptide antibiotic

ACTIVITY TEST

Cathelicidin Antimicrobial Peptide (CAMP) is a cationic host defense peptide predominantly expressed in neutrophils, epithelial cells, and keratinocytes, with a conserved cathelin domain at the N-terminus and a variable antimicrobial domain at the C-terminus. It exerts broad-spectrum antimicrobial activity against bacteria, fungi, and viruses by disrupting microbial cell membranes, and also modulates the host immune response by regulating cytokine secretion, promoting immune cell recruitment, and enhancing wound healing. CAMP plays a critical role in innate immunity, bridging the gap between innate and adaptive immune systems. Abnormal CAMP expression is associated with inflammatory disorders, skin diseases, and infectious pathologies, making it a promising target for developing antimicrobial therapeutics. CAMP interacts with lactoferrin (LTF) to synergistically enhance antimicrobial efficacy and immune regulatory functions.Thus a functional ELISA assay was conducted to detect the interaction of recombinant mouse CAMP and recombinant human LTF. Briefly, CAMP was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to LTF-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti-CAMP pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37℃, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 µL stop solution to the wells and read at 450/630nm immediately. The binding activity of recombinant mouse CAMP and recombinant human LTF was shown in Figure 1, the EC50 for this effect is 0.612µg/mL.

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
University of Waikato The Assessment of Vitamin D, Antimicrobial Peptides and Procalcitonin in Bronchiectasis Waikato:Source
Clin Infect Dis. Host immune response to tuberculous meningitis Pubmed:25301213
Vaccine On birth single dose live attenuated OPV and BCG vaccination induces gut cathelicidin LL37 responses at 6 week of age: A natural experiment Pubmed:25444792
Urology Modulating the Internalization of Bacille Calmette-Guérin by Cathelicidin in Bladder Cancer Cells Pubmed:25681250
Urology Modulating the Internalization of Bacille Calmette-Guérin by Cathelicidin in Bladder Cancer Cells PubMed: 25681250
Sarcoidosis Vasc Diffuse Lung Dis Cathelicidin as a link between sarcoidosis and tuberculosis PubMed: 26422567
Allergy and Asthma Proceedings The association of vitamin D, cathelicidin, and vitamin D binding protein with acute asthma attacks in children PubMed: 26108071
J Clin Microbiol. Swiftly Decreasing Cerebrospinal Fluid Cathelicidin Concentration Predicts Improved Outcome in Childhood Bacterial Meningitis. Pubmed:27008883
experimental dermatology Light-emitting diodes downregulate cathelicidin, kallikrein and toll-like receptor 2 expressions in keratinocytes and rosacea-like mouse skin. pubmed:27315464
PLOS ONE Reduced Expression of the Extracellular Calcium-Sensing Receptor (CaSR) Is Associated withActivation of the Renin-Angiotensin System (RAS) to Promote Vascular Remodeling in the Pathogenesis of Essential Hypertension. pubmed:27391973
Front Pharmacol.  Ginsenoside Rg5 Inhibits Succinate-Associated Lipolysis in Adipose Tissue and Prevents Muscle Insulin Resistance. pubmed:28261091
Allergologia et immunopathologia The relation of innate and adaptive immunity with viral-induced acute asthma attacks: Focusing on IP-10 and cathelicidin pubmed:27955890
Chinese Journal of Osteoporosis and Bone Mineral Research Effects of cold exposure on the bone marrow adipose tissue DOI: 10.3969/j.issn.1674-2591.2017.05.008
Scientific Reports Measurements of AMPs in stratum corneum of atopic dermatitis and healthy skin–tape stripping technique Pubmed:29374283
Experimental Parasitology Antimalarial activity of vitamin D3 (VD3) does not result from VD3-induced antimicrobial agents including nitric oxide or cathelicidin Pubmed: 30904694
Theranostics Myeloid cell-derived LL-37 promotes lung cancer growth by activating Wnt/β-catenin signaling Pubmed: 31149039
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES Short-Term versus Long-Term Culture of A549 Cells for Evaluating the Effects of Lipopolysaccharide on Oxidative Stress, Surfactant Proteins and Cathelicidin LL-37 Pubmed: 32050475
MICROB PATHOGENESIS Hypovitaminosis D and reduced cathelicidin are strongly correlated during the multidrug therapy against leprosy Pubmed: 32645421
International Journal of Nanomedicine Effect of Lonicerae japonicae Flos Carbonisata-Derived Carbon Dots on Rat Models of Fever and Hypothermia Induced by Lipopolysaccharide Pubmed: 32606669
博士论文 Relações causais entre a expressão dos genes do receptor de Vitamina D e do Peptídeo Antimicrobiano Catelicidina sobre marcadores sorológicos de pessoas com …
PLoS One Beneficial impact of cathelicidin on hypersensitivity pneumonitis treatment¡ªIn vivo studies 33999928
New Med Analysis of the level of non-specific and specific immunity parameters in saliva of children with osteogenesis imperfecta and study of relationships between?¡­
Type I interferons link skin-associated dysbiotic commensal bacteria to pathogenic inflammation and angiogenesis in rosacea
Int J Mol Sci Compartmentalized Innate Immune Response of Human Fetal Membranes against Escherichia coli Choriodecidual Infection Pubmed:35328414
Cell Rep LL-37 transports immunoreactive cGAMP to activate STING signaling and enhance interferon-mediated host antiviral immunity Pubmed:35649354
Catalog No. Related products for research use of Mus musculus (Mouse) Organism species Applications (RESEARCH USE ONLY!)
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