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Active Carnitine Palmitoyltransferase 2, Mitochondrial (CPT2)

CPTASE; Carnitine O-Palmitoyltransferase 2; Carnitine palmitoyltransferase II

ACTIVITY TEST

Carnitine Palmitoyltransferase 2(CPT2) is an enzyme that participates in fatty acid oxidation. It plays a crucial role in the transport of long-chain fatty acids into the mitochondria for beta-oxidation, which is the process that the body uses to break down these fatty acids to produce energy.Besides,Carnitine Palmitoyltransferase 1A(CPT1A) has been identified as an interactor of CPT2, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant rat CPT2 and recombinant human CPT1A. Briefly, CPT2 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to CPT1A-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti-CPT2 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37℃, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 µL stop solution to the wells and read at 450/630nm immediately. The binding activity of recombinant rat CPT2 and recombinant human CPT1A was shown in Figure 1, The binding activity of 777 and 000 was shown in Figure 1, and this effect was in a dose dependent manner.

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
Journal of the Neurological Sciences Normal protein content but abnormally inhibited enzyme activity in muscle carnitine palmitoyltransferase II deficiency ScienceDirect: S0022510X14000975
World journal of gastroenterology Mitochondrial carnitine palmitoyl transferase-II inactivity aggravates lipid accumulation in rat hepatocarcinogenesis. pubmed:28127199
Catalog No. Related products for research use of Rattus norvegicus (Rat) Organism species Applications (RESEARCH USE ONLY!)
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