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Active Carboxylesterase 1 (CES1)

ACAT; CEH; CES2; HMSE1; PCE1; SES1; TGH; Retinyl ester hydrolase; Serine esterase 1; Triacylglycerol hydrolase; Egasyn; Cocaine carboxylesterase; Liver Carboxylesterase 1

ACTIVITY TEST

carboxylesterase 1(CES1) also known as Liver carboxylesterase 1 is a serine esterase and member of a large multigene carboxylesterase family. The protein Involved in the detoxification of xenobiotics and in the activation of ester and amide prodrugs. Hydrolyzes aromatic and aliphatic esters, but has no catalytic activity toward amides or a fatty acyl-CoA ester. Hydrolyzes the methyl ester group of cocaine to form benzoylecgonine. Thus, the recombinant mouse CES1 activity was measured by its ability to hydrolyze 4-Nitrophenyl acetate (4-NPA) to 4-Nitrophenol. The reaction was performed in 50 mM Tris, 150 mM NaCl, pH 7.5 (Assay Buffer), ainitiated by addition 50 μL of various concentrations of CES1 (diluted by Assay Buffer) to 50 µL of 2 mM Substrate 4-NPA (100 mM stock in Acetone, diluted by Assay Buffer). Incubated at 37℃ for 10min, then read at a wavelength of 400 nm.

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
International Journal of Nanomedicine Gold Nanoparticles Perturb Drug-Metabolizing Enzymes and Antioxidants in the Livers of Male Rats: Potential Impact on Drug Interactions Pubmed: 32764932
Catalog No. Related products for research use of Mus musculus (Mouse) Organism species Applications (RESEARCH USE ONLY!)
APC374Mu02 Active Carboxylesterase 1 (CES1) Cell culture; Activity Assays.
APC374Mu61 Active Carboxylesterase 1 (CES1) Cell culture; Activity Assays.
EPC374Mu61 Eukaryotic Carboxylesterase 1 (CES1) Positive Control; Immunogen; SDS-PAGE; WB.
RPC374Mu02 Recombinant Carboxylesterase 1 (CES1) Positive Control; Immunogen; SDS-PAGE; WB.
APC374Mu01 Active Carboxylesterase 1 (CES1) Cell culture; Activity Assays.
RPC374Mu01 Recombinant Carboxylesterase 1 (CES1) Positive Control; Immunogen; SDS-PAGE; WB.
PAC374Mu01 Polyclonal Antibody to Carboxylesterase 1 (CES1) WB; IHC; ICC; IP.