≡

Active Transferrin (TF)

TRF; Siderophilin; Serotransferrin; Beta-1 metal-binding globulin

ACTIVITY TEST

Transferrin (TF) is one of members of anion-binding superfamily of proteins. It is synthesized as a 698 amino acid (aa) precursor, which is mainly in the liver and is present in high concentrations in the bloodstream.TF plays a fundamental role in iron metabolism, protecting tissues from the potentially harmful effects of free iron, which can generate reactive oxygen species and cause oxidative damage. To test the effect of TF on cell proliferation, HepG2 cells were seeded into triplicate wells of 96-well plates and allowed to attach, replaced with various concentrations of recombinant dog TF. After incubated for 72h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10 µl of CCK-8 solution was added to each well of the plate, then the absorbance at 450 nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37 ℃. Cell viability was assessed by CCK-8 assay after incubation with recombinant dog TF for 72h. The result was shown in Figure 1. It was obvious that TF significantly increased cell viability of HepG2 cells. The ED50 of recombinant dog TF is 0.292 μg/ml.

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
PLoS ONE Identification of Altered Plasma Proteins by Proteomic Study in Valvular Heart Diseases and the Potential Clinical Significance PubMed: PMC3754973
Physiological research The Labile Iron Pool in Monocytes Reflects the Activity of the Atherosclerotic Process in Men with Chronic Cardiovascular Disease pubmed:27782743
Journal of Proteomics Putative salivary biomarkers useful to differentiate patients with fibromyalgia Pubmed:29654921
Journal of Clinical Medicine Urinary Proteomics for the Early Diagnosis of Diabetic Nephropathy in Taiwanese Patients Pubmed: 30486327
The Nigerian postgraduate medical journal the official publication of the National Postgraduate Medical College of Nigeria Assessment of iron deficiency anaemia and its risk factors among adults with chronic kidney disease in a tertiary hospital in Nigeria
Haematologica New thiazolidinones reduce iron overload in mouse models of hereditary hemochromatosis and β-thalassemia Pubmed: 30792208
Catalog No. Related products for research use of Canis familiaris; Canine (Dog) Organism species Applications (RESEARCH USE ONLY!)
RPC036Ca02 Recombinant Transferrin (TF) Positive Control; Immunogen; SDS-PAGE; WB.
RPC036Ca03 Recombinant Transferrin (TF) Positive Control; Immunogen; SDS-PAGE; WB.
EPC036Ca61 Eukaryotic Transferrin (TF) Positive Control; Immunogen; SDS-PAGE; WB.
APC036Ca61 Active Transferrin (TF) Cell culture; Activity Assays.
RPC036Ca01 Recombinant Transferrin (TF) Positive Control; Immunogen; SDS-PAGE; WB.
PAC036Ca01 Polyclonal Antibody to Transferrin (TF) WB,IHC
MAC036Ca21 Monoclonal Antibody to Transferrin (TF) WB; IHC; ICC; IP.
SEC036Ca ELISA Kit for Transferrin (TF) Enzyme-linked immunosorbent assay for Antigen Detection.
SCC036Ca CLIA Kit for Transferrin (TF) Chemiluminescent immunoassay for Antigen Detection.
LMC036Ca Multiplex Assay Kit for Transferrin (TF) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.