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Active Serpin A10 (SERPINA10)

PZI; ZPI; Serine(Cysteine)Proteinase Inhibitor,Clade A(Alpha-1 Antiproteinase,Antitrypsin)Member 10; Protein Z-Related Protease Inhibitor; Protein Z Dependent Protease Inhibitor

ACTIVITY TEST

Protein Z-dependent Protease Inhibitor (ZPI), also known as SerpinA10 (SERine Proteinase INhibitor-clade A10) is a monomeric, secreted member of the A (or extracellular) clade within the serpin superfamily of protease inhibitors. In general, members of this superfamily regulate multiple proteolytic cascades, and are particularly effective due to the fact that their inhibitory activities can be fine-tuned through the participation of discrete, non-serpin co-factors. Serpins are unusual in that they are one-time use, non-recyclable proteins whose native state is thermodynamically unstable. The activity of recombinant human SERPINA10 was measured by its ability to inhibit Coagulation Factor X cleavage of a fluorogenic peptide substrate Mca-RPKPVE-Nval-WRK(Dnp)-NH2 in the assay buffer 50 mM Tris, 10 mM CaCl2, 150 mM NaCl, 0.05% (w/v) Brij-35, pH 7.5. Coagulation Factor X was diluted to 5 ug/ml in the assay buffer and 25 ul different concentrations of recombinant human SERPINA10 (MW: 78.26 KD) was incubated with 25 ul diluted Coagulation Factor X at 37 ℃ for 30 minutes. Loading 50 µL 20 µM substrate to start the reaction including a substrate blank containing 50 µL of assay buffer and 50 µL of 20 µM substrate. Then read at excitiation and emission wavelengths of 320 nm and 405 nm, respectively, in kinetic mode for 5 minutes. The result was shown in Figure 1 and it was obvious that recombinant human SERPINA10 significantly decreased Coagulation Factor X activity. The inhibition IC50 was <75 nM.

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
Thrombosis Research Plasma concentration of protein Z and protein Z-dependent protease inhibitor in patients with haemophilia A PubMed: 23269381
Clin Appl Thromb Hemost Plasma Concentrations of Protein Z and Protein Z-Dependent Protease Inhibitor in Patients With Essential Thrombocythemia PubMed: 25783867
Angiology Assessment of the Procoagulant Activity of Microparticles and the Protein Z System in Patients Undergoing Off-Pump Coronary Artery Bypass Surgery. pubmed:28464697
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
RPC010Hu02 Recombinant Serpin A10 (SERPINA10) Positive Control; Immunogen; SDS-PAGE; WB.
APC010Hu01 Active Serpin A10 (SERPINA10) Cell culture; Activity Assays.
RPC010Hu01 Recombinant Serpin A10 (SERPINA10) Positive Control; Immunogen; SDS-PAGE; WB.
PAC010Hu01 Polyclonal Antibody to Serpin A10 (SERPINA10) WB; IHC; ICC; IP.
MAC010Hu21 Monoclonal Antibody to Serpin A10 (SERPINA10) WB
MAC010Hu23 Monoclonal Antibody to Serpin A10 (SERPINA10) WB; IHC; ICC; IP.
MAC010Hu27 Monoclonal Antibody to Serpin A10 (SERPINA10) WB; IHC; ICC; IP.
MAC010Hu25 Monoclonal Antibody to Serpin A10 (SERPINA10) WB; IHC; ICC; IP.
MAC010Hu29 Monoclonal Antibody to Serpin A10 (SERPINA10) WB; IHC; ICC; IP.
MAC010Hu22 Monoclonal Antibody to Serpin A10 (SERPINA10) WB; IHC
SEC010Hu ELISA Kit for Serpin A10 (SERPINA10) Enzyme-linked immunosorbent assay for Antigen Detection.
LMC010Hu Multiplex Assay Kit for Serpin A10 (SERPINA10) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.