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Active Interleukin 34 (IL34)

C16orf77

ACTIVITY TEST

Interleukin 34 (IL-34) is a protein belonging to a group of cytokines called interleukins. IL-34 increases growth or survival of immune cells known as monocytes; it elicits its activity by binding the Colony stimulating factor 1 receptor. To explore the effect of IL-34 induction of bone marrow cells differentiation into macrophages,1×106 density of bone marrow cells were cultured with different concentration of recombinant mouse IL-34 for 7days, every three day changed with fresh culture. Setting the blank well without IL-34 as the control well. After 7days later, bone marrow cells culture with 1ug/ml IL-34 were significantiy differentiation into macrophages. The result observed by inverted microscope was shown on Figure1. Ink phagocytosis test shows ,after stimulated by IL-34, most of cells have phagocytosis function. The results were shown on Figure2.

Figure2. Ink phagocytosis test.

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
J Int Med Res. Increased Serum Interleukin-34 in Patients with Coronary Artery Disease PubMed: 23206468
Transplantation IL-34 inhibits acute rejection of rat liver transplantation by inducing Kupffer cell M2 polarization Pubmed:29570162
cyberleninka КЛИНИКО-ЭПИДЕМИОЛОГИЧЕСКАЯ ХАРАКТЕРИСТИКА И ОСОБЕННОСТИ БАЛЛАНСА ЦИТОКИНОВ ПРИ КЛЕЩЕВОМ ЭНЦЕФАЛИТЕ В …
Revista da Associacao Medica Brasileira A comparison of IL-17 and IL-34 concentrations in the cerebrospinal fluid of patients with acute inflammatory demyelinating neuropathy and chronic …
Rev Assoc Med Bras A comparison of IL-17 and IL-34 concentrations in the cerebrospinal fluid of patients with acute inflammatory demyelinating neuropathy and chronic?¡­ 33295414
Catalog No. Related products for research use of Mus musculus (Mouse) Organism species Applications (RESEARCH USE ONLY!)
RPC007Mu02 Recombinant Interleukin 34 (IL34) Positive Control; Immunogen; SDS-PAGE; WB.
APC007Mu61 Active Interleukin 34 (IL34) Cell culture; Activity Assays.
EPC007Mu61 Eukaryotic Interleukin 34 (IL34) Positive Control; Immunogen; SDS-PAGE; WB.
RPC007Mu01 Recombinant Interleukin 34 (IL34) Positive Control; Immunogen; SDS-PAGE; WB.
APC007Mu01 Active Interleukin 34 (IL34) Cell culture; Activity Assays.
PAC007Mu01 Polyclonal Antibody to Interleukin 34 (IL34) WB; IHC; ICC; IP.
PAC007Mu02 Polyclonal Antibody to Interleukin 34 (IL34) WB; IHC; ICC; IP.
LAC007Mu71 Biotin-Linked Polyclonal Antibody to Interleukin 34 (IL34) WB; IHC; ICC.
MAC007Mu21 Monoclonal Antibody to Interleukin 34 (IL34) WB; IHC; ICC; IP.
MEC007Mu Mini Samples ELISA Kit for Interleukin 34 (IL34) Enzyme-linked immunosorbent assay for Antigen Detection.
SEC007Mu ELISA Kit for Interleukin 34 (IL34) Enzyme-linked immunosorbent assay for Antigen Detection.
SCC007Mu CLIA Kit for Interleukin 34 (IL34) Chemiluminescent immunoassay for Antigen Detection.
LMC007Mu Multiplex Assay Kit for Interleukin 34 (IL34) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.