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Active Interleukin 34 (IL34)

C16orf77

ACTIVITY TEST

Interleukin-34 (IL-34) is a cytokine that binds to colony-stimulating factor-1 receptor (CSF-1R) and plays crucial roles in monocyte survival, differentiation, and osteoclastogenesis. It is also involved in inflammatory responses, autoimmune diseases, and tumor microenvironment regulation. IL-34 is produced by various cell types, including keratinocytes, neurons, and endothelial cells, and has been implicated in pathological conditions such as rheumatoid arthritis, neurodegenerative disorders, and cancer progression.Besides,Signal Transducer And Activator Of Transcription 3 (STAT3) has been identified as an interactor of IL-34, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human IL-34 and recombinant human STAT3 . Briefly, biotin-linked IL-34 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100μl were then transferred to STAT3-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST 3 times and incubation with Streptavidin-HRP for 30min, then wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µl stop solution to the wells and read at 450nm immediately. The binding activity of IL-34 and STAT3 was shown in Figure 1, the EC50 for this effect is 0.05µg/mL.

USAGE

Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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INCREMENT SERVICES

Magazine Citations
J Int Med Res. Increased Serum Interleukin-34 in Patients with Coronary Artery Disease PubMed: 23206468
Transplantation IL-34 inhibits acute rejection of rat liver transplantation by inducing Kupffer cell M2 polarization Pubmed:29570162
cyberleninka КЛИНИКО-ЭПИДЕМИОЛОГИЧЕСКАЯ ХАРАКТЕРИСТИКА И ОСОБЕННОСТИ БАЛЛАНСА ЦИТОКИНОВ ПРИ КЛЕЩЕВОМ ЭНЦЕФАЛИТЕ В …
Revista da Associacao Medica Brasileira A comparison of IL-17 and IL-34 concentrations in the cerebrospinal fluid of patients with acute inflammatory demyelinating neuropathy and chronic …
Rev Assoc Med Bras A comparison of IL-17 and IL-34 concentrations in the cerebrospinal fluid of patients with acute inflammatory demyelinating neuropathy and chronic?¡­ 33295414
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
APC007Hu62 Active Interleukin 34 (IL34) Cell culture; Activity Assays.
APC007Hu61 Active Interleukin 34 (IL34) Cell culture; Activity Assays.
APC007Hu01 Active Interleukin 34 (IL34) Cell culture; Activity Assays.
RPC007Hu02 Recombinant Interleukin 34 (IL34) Positive Control; Immunogen; SDS-PAGE; WB.
APC007Hu63 Active Interleukin 34 (IL34) Cell culture; Activity Assays.
EPC007Hu62 Eukaryotic Interleukin 34 (IL34) Positive Control; Immunogen; SDS-PAGE; WB.
RPC007Hu01 Recombinant Interleukin 34 (IL34) Positive Control; Immunogen; SDS-PAGE; WB.
PAC007Hu01 Polyclonal Antibody to Interleukin 34 (IL34) WB; IHC; ICC; IP.
MAC007Hu21 Monoclonal Antibody to Interleukin 34 (IL34) WB
MAC007Hu23 Monoclonal Antibody to Interleukin 34 (IL34) WB
MAC007Hu22 Monoclonal Antibody to Interleukin 34 (IL34) WB
SEC007Hu ELISA Kit for Interleukin 34 (IL34) Enzyme-linked immunosorbent assay for Antigen Detection.
SCC007Hu CLIA Kit for Interleukin 34 (IL34) Chemiluminescent immunoassay for Antigen Detection.
LMC007Hu Multiplex Assay Kit for Interleukin 34 (IL34) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.