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Active Interleukin 33 (IL33)

DV27; C9ORF26; IL1F11; NFHEV; Interleukin-1 Family, Member 11; Nuclear factor from high endothelial venules

ACTIVITY TEST

Figure. The binding activity of IL33 with IL1RL1.
Interleukin 33 is a member of the IL-1 family that potently drives production of T helper-2 (Th2)-associated cytokines. IL33 is a ligand for ST2 (IL1RL1), an IL-1 family receptor that is highly expressed on Th2 cells, mast cells and group 2 innate lymphocytes. IL-33 is expressed by a wide variety of cell types, including fibroblasts, mast cells, dendritic cells, macrophages, osteoblasts, endothelial cells, and epithelial cells. Besides, Interleukin 1 Receptor Like Protein 1 (IL1RL1) has been identified as an interactor of IL33, thus a binding ELISA assay was conducted to detect the interaction of recombinant human IL33 and recombinant human IL1RL1. Briefly, IL33 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to IL1RL1-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-IL33pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of IL33and IL1RL1 was shown in Figure 1, and this effect was in a dose dependent manner.

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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INCREMENT SERVICES

Magazine Citations
The Korean Journal of Internal Medicine Interleukin-33, matrix metalloproteinase-9, and tissue ınhibitor of matrix metalloproteinase-1 in myocardial infarction PubMed: PMC3604606
Acta Histochem CXCL13 blockade attenuates lupus nephritis of MRL/lpr mice PubMed: 26456520
Journal of Interferon & Cytokine Research Epithelial Cell-Derived Cytokines Contribute to the Pathophysiology of Eosinophilic Chronic Rhinosinusitis Pubmed:26540312
Journal of Interferon & Cytokine Research The Expression and Regulation of Interleukin-33 in Human Epidermal Keratinocytes: A New Mediator of Atopic Dermatitis and Its Possible Signaling Pathway. pubmed:27348082
Archives of Dermatological Research IL-33 circulating serum levels are increased in patients with non-segmental generalized vitiligo pubmed:27388717
Sci Pharm. Chrysin Protects Rat Kidney from Paracetamol-Induced Oxidative Stress, Inflammation, Apoptosis, and Autophagy: A Multi-Biomarker Approach. pubmed:28134775
Biotechnology & Biotechnological Equipment  Association of IL-33, IL1RL1 gene polymorphisms with serum IL-33 levels and risk of asthma in adults and asthmatic bronchitis in children (Chinese) 10.1080:13102818.2018.1471361
Tropical Journal of Pharmaceutical Research Role of IL-33 and ST2 signaling and inflammatory responses in non-small cell lung cancer 172341
Particle and Fibre Toxicology Group II innate lymphoid cells and microvascular dysfunction from pulmonary titanium dioxide nanoparticle exposure Pubmed: 30413212
Scientific Reports Interleukin-33 serum levels in postmenopausal women with osteoporosis Pubmed: 30846811
Cell Reports Tumor-Derived Lactic Acid Contributes to the Paucity of Intratumoral ILC2s Pubmed: 32101749
Allergy Asthma & Immunology Research Relationship of microbial profile with airway immune response in eosinophilic or neutrophilic inflammation of asthmatics Pubmed: 32141256
Traditional Medicine Research Qingfei oral liquid downregulates TRPV1 expression to reduce airway inflammation and mucus hypersecretion injury caused by respiratory syncytial virus infection …
Aggravation of Airway Inflammation in RSV-Infected Asthmatic Mice Following Infection-Induced Alteration of Gut Microbiota
J Immunol Res Inhibition of NF-κB/IL-33/ST2 Axis Ameliorates Acute Bronchiolitis Induced by Respiratory Syncytial Virus 34395633
Pathogens Elevated Levels of IL-33, IL-17 and IL-25 Indicate the Progression from Chronicity to Hepatocellular Carcinoma in Hepatitis C Virus Patients Pubmed:35056005
researchsquare Dexmedetomidine Attenuates Hyperalgesia Induced By Brachial Plexus Root Avulsion By Restoring The GLT-1 Function Via PKA Signaling
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
RPB980Hu02 Recombinant Interleukin 33 (IL33) Positive Control; Immunogen; SDS-PAGE; WB.
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RPB980Hu01 Recombinant Interleukin 33 (IL33) Positive Control; Immunogen; SDS-PAGE; WB.
PAB980Hu02 Polyclonal Antibody to Interleukin 33 (IL33) WB; IHC; ICC; IP.
PAB980Hu01 Polyclonal Antibody to Interleukin 33 (IL33) WB; IHC; ICC; IP.
LAB980Hu71 Biotin-Linked Polyclonal Antibody to Interleukin 33 (IL33) WB; IHC; ICC.
RAB980Hu21 Recombinant Antibody to Interleukin 33 (IL33) WB; IF; ICC; IHC; IP; FCM.
MAB980Hu24 Monoclonal Antibody to Interleukin 33 (IL33) WB; IHC; ICC; IP.
MAB980Hu26 Monoclonal Antibody to Interleukin 33 (IL33) WB; IHC; ICC; IP.
MAB980Hu21 Monoclonal Antibody to Interleukin 33 (IL33) WB; IHC; ICC; IP.
MAB980Hu22 Monoclonal Antibody to Interleukin 33 (IL33) WB; IHC
LAB980Hu72 Biotin-Linked Monoclonal Antibody to Interleukin 33 (IL33) WB; IHC; ICC.
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SEB980Hu ELISA Kit for Interleukin 33 (IL33) Enzyme-linked immunosorbent assay for Antigen Detection.
SCB980Hu CLIA Kit for Interleukin 33 (IL33) Chemiluminescent immunoassay for Antigen Detection.
LMB980Hu Multiplex Assay Kit for Interleukin 33 (IL33) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
PSB980Hu01 Antibody Pair for Interleukin 33 (IL33) ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays.
KSB980Hu01 ELISA Kit DIY Materials for Interleukin 33 (IL33) Main materials for "Do It (ELISA Kit) Yourself".