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Active Calpain 1 (CAPN1)

PIG30; CANPL1; MuCANP; MuCL; Calpain 1, Large Subunit; Calpain-1 Catalytic Subunit; Calcium-Dependent Protease 1; Calcium-activated neutral proteinase 1; Calpain mu-type; Micromolar-calpain

ACTIVITY TEST

Calpain 1, Large Subunit (CAPN1) is an intracellular protease that requires calcium for its catalytic activity. Calcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction. It has broad endopeptidase specificity. Besides, Signal Transducer And Activator Of Transcription 3 (STAT3) has been identified as an interactor of CAPN1, thus a binding ELISA assay was conducted to detect the interaction of recombinant rat CAPN1 and recombinant rat STAT3. Briefly, CAPN1 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to STAT3-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-CAPN1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of of CAPN1 and STAT3 was shown in Figure 1, and this effect was in a dose dependent manner.

USAGE

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
Bulletin du Cancer Calpain 1 in bronchoalveolar lavage fluid is associated with poor prognosis in lepidic predominant pulmonary adenocarcinoma Pubmed: 30683309
Catalog No. Related products for research use of Rattus norvegicus (Rat) Organism species Applications (RESEARCH USE ONLY!)
APB964Ra01 Active Calpain 1 (CAPN1) Cell culture; Activity Assays.
APB964Ra02 Active Calpain 1 (CAPN1) Cell culture; Activity Assays.
RPB964Ra01 Recombinant Calpain 1 (CAPN1) Positive Control; Immunogen; SDS-PAGE; WB.
RPB964Ra02 Recombinant Calpain 1 (CAPN1) Positive Control; Immunogen; SDS-PAGE; WB.
PAB964Ra01 Polyclonal Antibody to Calpain 1 (CAPN1) WB; IHC; ICC; IP.
PAB964Ra02 Polyclonal Antibody to Calpain 1 (CAPN1) WB; IHC; ICC; IP.
LAB964Ra71 Biotin-Linked Polyclonal Antibody to Calpain 1 (CAPN1) WB; IHC; ICC.
MAB964Ra21 Monoclonal Antibody to Calpain 1 (CAPN1) WB; IHC; ICC; IP.
SEB964Ra ELISA Kit for Calpain 1 (CAPN1) Enzyme-linked immunosorbent assay for Antigen Detection.
LMB964Ra Multiplex Assay Kit for Calpain 1 (CAPN1) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.