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Active Superoxide Dismutase 1 (SOD1)

ALS; ALS1; IPOA; Superoxide Dismutase 1, Soluble; Amyotrophic Lateral Sclerosis 1,Adult; Superoxide dismutase [Cu-Zn]

ACTIVITY TEST

Extracellular superoxide dismutase [Cu-Zn] is an enzyme that in humans is encoded by the SOD3 gene. This gene encodes a member of the superoxide dismutase (SOD) protein family. SODs are antioxidant enzymes that catalyze the dismutation of two superoxide radicals into hydrogen peroxide and oxygen. Acroding to the report, in a weakly alkaline buffer solution (pH=8.2) with N-tris(hydroxymethyl)amino methane-HCL, pyrogallol can occur autoxidation in the air, then SOD can inhibit this reaction. Thus, we use this way to measued the activity of recombinant mouse SOD1.The reaction was performed in adding 15μl 5mmol/L pyrogallol to 900μl 50mmol/L Tris-HCl in 1.5ml cuvette(1.0 cm light path),rapidly mixing at 25 ℃,then read at 325nm(zero the spectrophotometer using 50mmol/L Tris-HCl) , reacod the OD value every 30 second for 6 times. Control the pyrogallol autoxidation rate at 0.70 OD/min. After, adding various concentrations of recombinant of SOD1 to 900μl 50mmol/L Tris-HCl, incubated for 20min at 25 ℃, then adding 15μl 5mmol/L pyrogallol to each tube, rapidly mixing and read at 325nm, reacod the OD value every 30 second for 6 times. Under these conditions, the enzyme amount of 50% per minute inhibition of pyrogallol autooxidation is defined as a unit.

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
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Adv Med Sci. Orthodontic treatment modifies the oxidant-antioxidant balance in saliva of clinically healthy subjects. pubmed:28242484
Iranian Journal of Kidney Diseases Clinical Characterization of Serum Docosahexaenoic Acid and Its Relationship With Inflammation Factors in Patients With Diabetic Nephropathy Pubmed:29507271
Journal of Integrative Medicine Effects of Tualang honey in modulating nociceptive responses at the spinal cord in offspring of prenatally stressed rats Doi: 10.1016/j.joim.2018.12.002
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