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Active Annexin A2 (ANXA2)

ANX-A2; ANX2L4; CAL1H; LIP2; LPC2; LPC2D; P36; PAP-IV; Protein I; Chromobindin-8; Calpactin-1 heavy chain; Lipocortin II; Placental anticoagulant protein IV

ACTIVITY TEST

Figure. The binding activity of ANXA2 with S100A10.
Annexin A2 (ANXA2) also known as annexin II is a member of the annexin family. Members of this calcium-dependent phospholipid-binding protein family play a role in the regulation of cellular growth and in signal transduction pathways. This protein functions as an autocrine factor which heightens osteoclast formation and bone resorption. It is a calcium-dependent phospholipid-binding protein whose function is to help organize exocytosis of intracellular proteins to the extracellular domain. Besides, S100 Calcium Binding Protein A10 (S100A10) has been identified as an interactor of ANXA2, thus a binding ELISA assay was conducted to detect the interaction of recombinant mouse ANXA2 and recombinant mouse S100A10. Briefly, ANXA2 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to S100A10-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-ANXA2 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of ANXA2 and S100A10 was shown in Figure 1, and this effect was in a dose dependent manner.

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
Journal of Cellular Biochemistry Subcellular proteome analysis unraveled annexin A2 related to immune liver fibrosis PubMed: 20225235
World Journal of Gastroenterology Expression characteristics and diagnostic value of annexin A2 in hepatocellular carcinoma PubMed: PMC3491596
Oncology Letters Annexin A2 is not a good biomarker for hepatocellular carcinoma in cirrhosis PubMed: PMC3742823
Ibrahim Annexin A2 Versus Afp as An Efficient Diagnostic Serum Marker for Hepatocellular Carcinoma Ghrnet: Source
European Journal of Clinical Microbiology & Infectious Diseases Serum annexin A2 levels in acute brucellosis and brucellar spondylodiscitis Pubmed:24853056
Biomedicine & Pharmacotherapy Circulating annexin A2 as a biomarker in gastric cancer patients: Correlation with clinical variables Pubmed:25661364
Hepat Mon Serum Levels of Annexin A2 as a Candidate Biomarker for Hepatic Fibrosis in Patients With Chronic Hepatitis B PubMed: 26587036
Tumour Biol Up-regulation of annexin A2 expression predicates advanced clinicopathological features and poor prognosis in hepatocellular carcinoma PubMed: 26109000
Biomed Pharmacother Circulating PubMed: 25661364
Tumour Biol Evaluation of annexin A2 and as potential biomarkers for hepatocellular carcinoma PubMed: 26189841
Eur J Clin Invest Stairs instead of elevators at the workplace decreases PCSK9 levels in a healthy population PubMed: 26081791
Journal of Oral and Maxillofacial Surgery Serum Annexin A2 Level Is Associated With Diagnosis and Prognosis in Patients With OralSquamous Cell Carcinoma. pubmed:27889534
Pregnancy Hypertens Assessment of annexin A5 and annexin A2 levels as biomarkers for pre-eclampsia: A pilot study. pubmed:28501283
Mol Nutr Food Res. Epigallocatechin gallate induces an up-regulation of LDL receptor accompanied by a reduction of PCSK9 via the annexin A2-independent pathway in HepG2 cells. pubmed:28181408
Archives of Gynecology and Obstetrics Expression of annexin A2 in adenomyosis and dysmenorrhea Pubmed: 31183557
Cancers Extracellular Vesicles-Based Biomarkers Represent a Promising Liquid Biopsy in Endometrial Cancer Pubmed: 31842290
PLASMA BIOMARKER FOR OVARIAN CANCER
Diagnostics Diagnostic Value of Plasma Annexin A2 in Early-Stage High-Grade Serous Ovarian Cancer. Diagnostics 2021, 11, 69 33406648
ADVANCED RESEARCHES Search for Effective Serum Tumor Markers for Early Diagnosis of Hepatocellular Carcinoma Associated with Hepatitis C
Cancer Cell International Bufalin Induced Mitochondrial Dysfunction Promotes Apoptosis of Glioma Cells by Regulating Annexin A2 and DRP1 Proteins
Cancer Cell Int Bufalin induces mitochondrial dysfunction and promotes apoptosis of glioma cells by regulating Annexin A2 and DRP1 protein expression 34376212
Catalog No. Related products for research use of Mus musculus (Mouse) Organism species Applications (RESEARCH USE ONLY!)
RPB944Mu02 Recombinant Annexin A2 (ANXA2) Positive Control; Immunogen; SDS-PAGE; WB.
RPB944Mu01 Recombinant Annexin A2 (ANXA2) Positive Control; Immunogen; SDS-PAGE; WB.
APB944Mu01 Active Annexin A2 (ANXA2) Cell culture; Activity Assays.
PAB944Mu01 Polyclonal Antibody to Annexin A2 (ANXA2) WB; IHC; ICC; IP.
PAB944Mu02 Polyclonal Antibody to Annexin A2 (ANXA2) WB; IHC; ICC; IP.
SEB944Mu ELISA Kit for Annexin A2 (ANXA2) Enzyme-linked immunosorbent assay for Antigen Detection.
LMB944Mu Multiplex Assay Kit for Annexin A2 (ANXA2) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.