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Active Tissue Factor Pathway Inhibitor 2 (TFPI2)

PP5; Placental protein 5

ACTIVITY TEST

Tissue Factor Pathway Inhibitor 2 (TFPI2) takes part in the regulation of plasmin-mediated matrix remodeling. Inhibits trypsin, plasmin, factor VIIa/tissue factor and weakly factor Xa. TFPI2 doesn’t have any influence on thrombin. TFPI2 also can inhibit MMP activity, which can hydrolyze gelatin under certain conditions. Thus, the activity of TFPI2 can be measured by inhibit MMP-2 hydrolyze gelatin. Gelatin zymography is mainly used for the detection of the gelatinases, 2μg/mL was denatured by SDS loading buffer, electrophoresed through sodium dodecylsulphate–polyacrylamide gel (SDS–PAGE; 8% gels) containing gelatin (1mg/mL) with nonreducing conditions. After renaturation, incubate with various concentrations of recombinant mouse TFPI2, then staining with coomassie brilliant blue G250, active MMP-2 would hydrolyze gelatin nearby, which was indicated by the white binds on the gel; if the activity of MMP-2 inhibit by TFPI2, there was none white binds on the gel.The result was shown in figure 1.
As the figure 1 shown, MMP-2 can be inhibited by recombinant mouse TFPI2 at least 2.5μg/mL.

USAGE

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

GIVEAWAYS

INCREMENT SERVICES

Catalog No. Related products for research use of Mus musculus (Mouse) Organism species Applications (RESEARCH USE ONLY!)
RPB940Mu01 Recombinant Tissue Factor Pathway Inhibitor 2 (TFPI2) Positive Control; Immunogen; SDS-PAGE; WB.
APB940Mu01 Active Tissue Factor Pathway Inhibitor 2 (TFPI2) Cell culture; Activity Assays.
PAB940Mu01 Polyclonal Antibody to Tissue Factor Pathway Inhibitor 2 (TFPI2) WB; IHC; ICC; IP.
SEB940Mu ELISA Kit for Tissue Factor Pathway Inhibitor 2 (TFPI2) Enzyme-linked immunosorbent assay for Antigen Detection.
LMB940Mu Multiplex Assay Kit for Tissue Factor Pathway Inhibitor 2 (TFPI2) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
PGB940Mu01 Primer Pair for Tissue Factor Pathway Inhibitor 2 (TFPI2) PCR