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Active Cathepsin S (CTSS)

CTS-S

ACTIVITY TEST

Cathepsin S (CTSS) is a lysosomal cysteine protease of the papain family. It plays a major role in the processing of the MHC class IIassociated invariant chain. It has been implicated in the pathogenesis of several diseases such as Alzheimer’s disease and degenerative disorders associated with the cells of the mononuclear phagocytic system. Mouse Cathepsin S is synthesized as a preproenzyme of 340 amino acid residues consisting a signal peptide (residues 117), a pro region (residues 18122), and the mature enzyme (residues 123340). Cathepsin S is less abundant in tissues than Cathepsins B, L and H. The activity of recombinant mouse CTSS is measured by its ability to cleave a fluorogenic peptide substrate Mca-Arg-Pro-Lys-Pro-Val-Glu-Nval-Trp-Arg-Lys(Dnp)-NH2 in the assay buffer 50 mM NaOAc, 5 mM DTT, 250 mM NaCl, pH 4.5. The rmCTSS is diluted to 50 ug/ml in assay buffer, then incubated at room temperature for 2h. The incubated rmCTSS is diluted to 5 ug/mL in assay buffer. Loading into a black well plate 50 µL of 5 ug/mL rmCTSS and start the reaction by adding 50 µL of 20 µM substrate, with a substrate blank containing 50 µL assay buffer, 50 µL substrate, and no rmCTSS. Then read at excitiation and emission wavelengths of 320 nm and 405 nm, respectively, in kinetic mode for 5 minutes. The specific activity of recombinant mouse CTSS is > 10 pmol/min/µg.

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
Chinses Medical Journal Relationship between plasma cathepsin S and cystatin C levels and coronary plaque morphology of mild to moderate lesions: an in vivo study using intravascular ultrasound CMJ: 2009124346863507950
Journal of Cystic Fibrosis Protease–antiprotease imbalances differ between Cystic Fibrosis patients' upper and lower airway secretions Pubmed:25286826
Scientific Reports Circulating cathepsin-S levels correlate with GFR decline and sTNFR1 and sTNFR2 levels in mice and humans. pubmed:28240259
Neuroscience The P2X7 receptor, cathepsin S and fractalkine in the trigeminal subnucleus caudalis signal persistent hypernociception in temporomandibular rat joints Pubmed: 30248434
TOXICON Modulatory effect of botulinum toxin type A on the microglial P2X7/CatS/FKN activated-pathway in antigen-induced arthritis of the temporomandibular joint of … Pubmed: 32882256
Catalog No. Related products for research use of Mus musculus (Mouse) Organism species Applications (RESEARCH USE ONLY!)
RPB933Mu02 Recombinant Cathepsin S (CTSS) Positive Control; Immunogen; SDS-PAGE; WB.
RPB933Mu01 Recombinant Cathepsin S (CTSS) Positive Control; Immunogen; SDS-PAGE; WB.
APB933Mu01 Active Cathepsin S (CTSS) Cell culture; Activity Assays.
PAB933Mu01 Polyclonal Antibody to Cathepsin S (CTSS) WB; IHC; ICC; IP.
PAB933Mu02 Polyclonal Antibody to Cathepsin S (CTSS) WB; IHC; ICC; IP.
LAB933Mu81 FITC-Linked Polyclonal Antibody to Cathepsin S (CTSS) WB; IHC; ICC; IF.
LAB933Mu71 Biotin-Linked Polyclonal Antibody to Cathepsin S (CTSS) WB; IHC; ICC.
SEB933Mu ELISA Kit for Cathepsin S (CTSS) Enzyme-linked immunosorbent assay for Antigen Detection.
LMB933Mu Multiplex Assay Kit for Cathepsin S (CTSS) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.