Packages (Simulation)
Image (I)
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Figure . Gene Sequencing (extract)
Image (II)
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Figure. SDS-PAGE
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Instruction Manual
Certificate
Active Fibroblast Growth Factor 10 (FGF10)
Keratinocyte growth factor 2
- Product No.APB882Hu01
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Buffer Formulation20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
- Traits Freeze-dried powder
- Purity> 90%
- Isoelectric Point10.0
- ApplicationsCell culture; Activity Assays.
- DownloadInstruction Manual
- UOM 10µg50µg 200µg 1mg 5mg
- FOB
US$ 167
US$ 418
US$ 836
US$ 2508
US$ 6270
For more details, please contact local distributors!
ACTIVITY TEST
Fibroblast Growth Factor 10(FGF10)is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. Besides, Fibroblast Growth Factor Receptor 2 (FGFR2) has been identified as an interactor of FGF10, thus a binding ELISA assay was conducted to detect the interaction of recombinant human FGF10 and recombinant human FGFR2. Briefly, FGF10 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100μl were then transferred to FGFR2-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-FGF10 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µl stop solution to the wells and read at 450nm immediately. The binding activity of FGF10 and FGFR2 was shown in Figure 1, and this effect was in a dose dependent manner.
USAGE
Reconstitute in 20mM Tris, 150mM NaCl (PH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
STORAGE
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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| Magazine | Citations |
| Inflammation | Involvements of γδT Lymphocytes in Acute and Chronic Skin Wound Repair. pubmed:28540539 |
| Journal of Molecular Neuroscience | Dysregulation of Fibroblast Growth Factor 10 in the Peripheral Blood of Patients with Schizophrenia Pubmed: 31256336 |