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Active Nuclear Factor Kappa B2 (NFkB2)

LYT-10; H2TF1; Lymphocyte translocation chromosome 10; Nuclear Factor Of Kappa Light Polypeptide Gene Enhancer In B-Cells 2(p49/P100); Nuclear factor NF-kappa-B p52

ACTIVITY TEST

Nuclear Factor kappa B2 (NFkB2), also known as p100, is a subunit of the NF-kappa B (NFkB) family of transcription factors. NFkB2 plays an important role in immune response and inflammation. It is involved in regulating the expression of a variety of genes, including those encoding cytokines, chemokines, adhesion molecules, and acute phase response proteins. RELB-NFkB2 heterodimer regulates the expression of genes associated with immune response and inflammation in cells. Thus a functional binding ELISA assay was conducted to detect the interaction of recombinant mouse NFkB2 and recombinant human RELB. Briefly, NFkB2 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to RELB-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti-NFkB2 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37℃, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 µL stop solution to the wells and read at 450/630 nm immediately. The binding activity of recombinant mouse NFkB2 and recombinant human RELB was shown in Figure 1, the EC50 for this effect is 0.23 ug/mL.

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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