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Active Interleukin 25 (IL25)

IL17E; IL17-E; Interleukin-17E

ACTIVITY TEST

Interleukin-25 (IL-25) is a cytokine belonging to the IL-17 family, also known as IL-17E. It is primarily secreted by Th2 cells and mast cells, playing a key role in promoting type 2 immune responses. IL-25 induces the production of cytokines such as IL-4, IL-5, and IL-13, stimulating eosinophil expansion and contributing to allergic inflammation and anti-parasitic defenses.IL - 25 binds to a heterodimeric receptor complex composed of IL - 17RA and IL - 17RB. This binding initiates signaling cascades that lead to the activation of downstream immune - related pathways.Thus a functional binding ELISA assay was conducted to detect the interaction of recombinant rat IL-25 and recombinant human IL17RA .Briefly, biotin-linked IL-25 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100μl were then transferred to IL17RA-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST 3 times and incubation with Streptavidin-HRP for 30min, then wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µl stop solution to the wells and read at 450nm immediately. The binding activity of recombinant rat IL-25 and recombinant human IL17RA was shown in Figure 1, the EC50 for this effect is 0.59ug/mL.

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
Am J Clin Exp Immunol. Molecular analysis of interleukin-25 exons 1 and 2 and its serum levels in Iranian patients with multiple sclerosis Pubmed:Pmc4138132
Journal of Interferon & Cytokine Research Epithelial Cell-Derived Cytokines Contribute to the Pathophysiology of Eosinophilic Chronic Rhinosinusitis Pubmed:26540312
Cellular & Molecular Immunology IL-25 stimulates M2 macrophage polarization and thereby promotes mitochondrial respiratory capacity and lipolysis in adipose tissues against obesity. pubmed:28194019
Allergology International The effect of calprotectin on TSLP and IL-25 production from airway epithelial cells pubmed:27475624
Clinical Immunology A mechanism of interleukin-25 production from airway epithelial cells induced by Japanese cedar pollen Pubmed:29402661
Clinical Immunology Toll-like receptor 9 ligands increase type I interferon induced B-cell activating factor expression in chronic rhinosinusitis with nasal polyposis Pubmed:30056130
cyberleninka КЛИНИКО-ЭПИДЕМИОЛОГИЧЕСКАЯ ХАРАКТЕРИСТИКА И ОСОБЕННОСТИ БАЛЛАНСА ЦИТОКИНОВ ПРИ КЛЕЩЕВОМ ЭНЦЕФАЛИТЕ В …
OncoTargets and Therapy effect of Th9/il-9 on the growth of gastric cancer in nude mice Pubmed: 30988627
Cancer Medicine IL‐25 promotes cisplatin resistance of lung cancer cells by activating NF‐κB signaling pathway to increase of major vault protein Pubmed: 31044552
Traditional Medicine Research Qingfei oral liquid downregulates TRPV1 expression to reduce airway inflammation and mucus hypersecretion injury caused by respiratory syncytial virus infection …
Aggravation of Airway Inflammation in RSV-Infected Asthmatic Mice Following Infection-Induced Alteration of Gut Microbiota
Pathogens Elevated Levels of IL-33, IL-17 and IL-25 Indicate the Progression from Chronicity to Hepatocellular Carcinoma in Hepatitis C Virus Patients Pubmed:35056005
Catalog No. Related products for research use of Rattus norvegicus (Rat) Organism species Applications (RESEARCH USE ONLY!)
APB694Ra61 Active Interleukin 25 (IL25) Cell culture; Activity Assays.
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RPB694Ra01 Recombinant Interleukin 25 (IL25) Positive Control; Immunogen; SDS-PAGE; WB.
PAB694Ra01 Polyclonal Antibody to Interleukin 25 (IL25) WB; IHC
MAB694Ra21 Monoclonal Antibody to Interleukin 25 (IL25) WB; IHC; ICC; IP.
SEB694Ra ELISA Kit for Interleukin 25 (IL25) Enzyme-linked immunosorbent assay for Antigen Detection.
SCB694Ra CLIA Kit for Interleukin 25 (IL25) Chemiluminescent immunoassay for Antigen Detection.
LMB694Ra Multiplex Assay Kit for Interleukin 25 (IL25) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.