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Active B-Lymphocyte Chemoattractant (BLC)

CXCL13; SCYB13; BCA1; BLR1L; ANGIE; ANGIE2; Chemokine(C-X-C-Motif)ligand 13; Small Inducible Cytokine B Subfamily(Cys-X-Cys Motif)Member 13

ACTIVITY TEST

Lymphocyte Chemoattractant 1 (BLC1) , also known as CXC chemokine ligand 13 (CXCL13) is a member of the CC chemokine family andis constitutively expressed in secondary lymphoid organs. . BLC1 functions by attracting B lymphocytes to sites of inflammation, thus facilitating the immune response against pathogens. The protein is involved in various physiological processes, including B cell migration, proliferation, and differentiation. Additionally, BLC1 has been associated with several diseases, including autoimmune disorders and certain types of cancer.Chemokine C-X-C-Motif Receptor 5 (CXCR5) is the cognate receptor for BLC1,, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant humant BLC1 and recombinant human CXCR5. Briefly,BLC1 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to CXCR5-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti-BLC1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37℃, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 µL stop solution to the wells and read at 450/630nm immediately. The binding activity of recombinant humant BLC1 and recombinant human CXCR5 was shown in Figure 1, the EC50 for this effect is 0.62ug/mL.

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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