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Active Cofilin 1 (CFL1)

CFL; p18; N-Cofilin; 18 kDa phosphoprotein; Cofilin 1, Non Muscle

ACTIVITY TEST

Figure. The binding activity of CFL1 with ACTb.
Cofilin 1 (non-muscle; n-cofilin), also known as CFL1, is a human gene, part of the ADF/cofilin family. Cofilin is a widely distributed intracellular actin-modulating protein that binds and depolymerizes filamentous F-actin and inhibits the polymerization of monomeric G-actin in a pH-dependent manner. It is involved in the translocation of actin-cofilin complex from cytoplasm to nucleus. Besides, Actin Beta (ACTb) has been identified as an interactor of CFL1, thus a binding ELISA assay was conducted to detect the interaction of recombinant human CFL1 and recombinant human ACTb. Briefly, CFL1 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to ACTb-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-CFL1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of CFL1 and ACTb was shown in Figure 1, and this effect was in a dose dependent manner.

USAGE

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
RPB559Hu01 Recombinant Cofilin 1 (CFL1) Positive Control; Immunogen; SDS-PAGE; WB.
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PAB559Hu01 Polyclonal Antibody to Cofilin 1 (CFL1) WB; IHC; ICC; IP.
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MAB559Hu21 Monoclonal Antibody to Cofilin 1 (CFL1) WB; IHC; ICC; IP.
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MAB559Hu27 Monoclonal Antibody to Cofilin 1 (CFL1) WB; IHC; ICC; IP.
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MAB559Hu25 Monoclonal Antibody to Cofilin 1 (CFL1) WB; IHC; ICC; IP.
SEB559Hu ELISA Kit for Cofilin 1 (CFL1) Enzyme-linked immunosorbent assay for Antigen Detection.
SCB559Hu CLIA Kit for Cofilin 1 (CFL1) Chemiluminescent immunoassay for Antigen Detection.
LMB559Hu Multiplex Assay Kit for Cofilin 1 (CFL1) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.