≡

Active Tumor Necrosis Factor Receptor Superfamily, Member 9 (TNFRSF9)

CD137; CDw137; 4-1BB; ILA; Induced By Lymphocyte Activation; T-cell antigen 4-1BB homolog

ACTIVITY TEST

TNFRSF9 (Tumor necrosis factor receptor superfamily member 9) is a member of the TNF-receptor superfamily. This receptor contributes to the clonal expansion, survival, and development of T cells. A binding ELISA assay was conducted to detect the interaction of TNFRSF9 and TNFa. Briefly, recombinant human TNFRSF9 were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to TNFa-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-TNFRSF9 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of TNFRSF9 and TNFa was shown in Figure 1, and this effect was in a dose dependent manner.

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

GIVEAWAYS

INCREMENT SERVICES

Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
APB527Hu01 Active Tumor Necrosis Factor Receptor Superfamily, Member 9 (TNFRSF9) Cell culture; Activity Assays.
RPB527Hu01 Recombinant Tumor Necrosis Factor Receptor Superfamily, Member 9 (TNFRSF9) Positive Control; Immunogen; SDS-PAGE; WB.
PAB527Hu01 Polyclonal Antibody to Tumor Necrosis Factor Receptor Superfamily, Member 9 (TNFRSF9) WB;FCM
FAB527Hu01 Anti-Tumor Necrosis Factor Receptor Superfamily, Member 9 (TNFRSF9) Polyclonal Antibody Flow cytometry.
LAB527Hu71 Biotin-Linked Polyclonal Antibody to Tumor Necrosis Factor Receptor Superfamily, Member 9 (TNFRSF9) WB; IHC; ICC.
RAB527Hu21 Recombinant Antibody to Tumor Necrosis Factor Receptor Superfamily, Member 9 (TNFRSF9) WB; IFC; IHC; IP.
SEB527Hu ELISA Kit for Tumor Necrosis Factor Receptor Superfamily, Member 9 (TNFRSF9) Enzyme-linked immunosorbent assay for Antigen Detection.
LMB527Hu Multiplex Assay Kit for Tumor Necrosis Factor Receptor Superfamily, Member 9 (TNFRSF9) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.