≡

Active Granulysin (GNLY)

NKG5; LAG-2; TLA519; T-Lymphocyte Activation Gene 519

ACTIVITY TEST

Granulysin (GNLY) is a member of the saposin-like protein (SAPLIP) family of membrane disrupting proteins. Granulysin is expressed in granules of natural killer and activated cytotoxic T cells. It exhibits cytolytic activity against intracellular or extracellular microbes and also tumors, either alone or in synergy with perforin. Human granulysin has structural similarity and 30 - 40% aa identity to granulysins and NK-lysins of other mammals such as bovine, porcine and canine; similar peptides in rodents have not been identified. The 15 kDa unglycosylated protein contains five helical domains; helix 2 and 3 contain 9 arginines and one cysteine critical for activity. The activity of GNLY is usually measured by a cell apoptosis assay using Jurkat cells. Jurkat cells were seeded into triplicate wells of 96-well plates at a density of 8,000 cells/well with 5% serum standard 1640 which contains various concentrations of recombinant human GM-CSF. After incubated for 3 days, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10 µl of CCK-8 solution was added to each well of the plate, then the absorbance at 450 nm was measured using a microplate reader after incubating the plate for 2-4 hours at 37 ℃. Apoptosis of Jurkat cells after incubation with GNLY for 3 days observed by inverted microscope was shown in Figure 1. Cell viability was assessed by CCK-8 (Cell Counting Kit-8 ) assay after incubation with recombinant human GNLY for 3 days. The result was shown in Figure 2. It was obvious that GNLY significantly decreased cell viability of Jurkat cells. The ED50 is 0.84 ug/ml.

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
Acta Haematologica Pretreatment Serum Level of 15-kDa Granulysin Might Have a Prognostic Value in Patients with Diffuse Large B Cell Lymphoma Karger: 327255
ДИНАМИЧЕСКИЕ ИЗМЕНЕНИЯ ГРАНУЛИЗИНА И КАТЕЛИЦИДИНА У ДЕТЕЙ И ПОДРОСТКОВ, БОЛЬНЫХ РАЗЛИЧНЫМИ ФОРМАМИ ТУБЕРКУЛЕЗА …
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
RPB517Hu01 Recombinant Granulysin (GNLY) Positive Control; Immunogen; SDS-PAGE; WB.
APB517Hu61 Active Granulysin (GNLY) Cell culture; Activity Assays.
EPB517Hu61 Eukaryotic Granulysin (GNLY) Positive Control; Immunogen; SDS-PAGE; WB.
PAB517Hu01 Polyclonal Antibody to Granulysin (GNLY) WB; IHC; ICC; IP.
MAB517Hu22 Monoclonal Antibody to Granulysin (GNLY) WB; IHC; ICC; IP.
SEB517Hu ELISA Kit for Granulysin (GNLY) Enzyme-linked immunosorbent assay for Antigen Detection.
CMB517Hu Multiplex Assay Kit for Granulysin (GNLY) ,etc. by CBA (Cytometric Bead Array) FLIA Kit for Antigen Detection.
LMB517Hu Multiplex Assay Kit for Granulysin (GNLY) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.