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Active Granulysin (GNLY)

NKG5; LAG-2; TLA519; T-Lymphocyte Activation Gene 519

ACTIVITY TEST

Granulysin (GNLY) is a member of the saposin-like protein (SAPLIP) family of membrane disrupting proteins. Granulysin is expressed in granules of natural killer and activated cytotoxic T cells. It exhibits cytolytic activity against intracellular or extracellular microbes and also tumors, either alone or in synergy with perforin. Human granulysin has structural similarity and 30 - 40% aa identity to granulysins and NK-lysins of other mammals such as bovine, porcine and canine; similar peptides in rodents have not been identified. The 15 kDa unglycosylated protein contains five helical domains; helix 2 and 3 contain 9 arginines and one cysteine critical for activity. The activity of GNLY is usually measured by a cell apoptosis assay using Jurkat cells. Jurkat cells were seeded into triplicate wells of 96-well plates at a density of 8,000 cells/well with 5% serum standard 1640 which contains various concentrations of recombinant human GM-CSF. After incubated for 3 days, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10 µl of CCK-8 solution was added to each well of the plate, then the absorbance at 450 nm was measured using a microplate reader after incubating the plate for 2-4 hours at 37 ℃. Apoptosis of Jurkat cells after incubation with GNLY for 3 days observed by inverted microscope was shown in Figure 1. Cell viability was assessed by CCK-8 (Cell Counting Kit-8 ) assay after incubation with recombinant human GNLY for 3 days. The result was shown in Figure 2. It was obvious that GNLY significantly decreased cell viability of Jurkat cells. The ED50 is 0.84 ug/ml.

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
Acta Haematologica Pretreatment Serum Level of 15-kDa Granulysin Might Have a Prognostic Value in Patients with Diffuse Large B Cell Lymphoma Karger: 327255
ДИНАМИЧЕСКИЕ ИЗМЕНЕНИЯ ГРАНУЛИЗИНА И КАТЕЛИЦИДИНА У ДЕТЕЙ И ПОДРОСТКОВ, БОЛЬНЫХ РАЗЛИЧНЫМИ ФОРМАМИ ТУБЕРКУЛЕЗА …
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
RPB517Hu01 Recombinant Granulysin (GNLY) Positive Control; Immunogen; SDS-PAGE; WB.
APB517Hu61 Active Granulysin (GNLY) Cell culture; Activity Assays.
EPB517Hu61 Eukaryotic Granulysin (GNLY) Positive Control; Immunogen; SDS-PAGE; WB.
PAB517Hu01 Polyclonal Antibody to Granulysin (GNLY) WB; IHC; ICC; IP.
MAB517Hu22 Monoclonal Antibody to Granulysin (GNLY) WB; IHC; ICC; IP.
SEB517Hu ELISA Kit for Granulysin (GNLY) Enzyme-linked immunosorbent assay for Antigen Detection.
LMB517Hu Multiplex Assay Kit for Granulysin (GNLY) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.