≡

Active Interleukin 5 Receptor Alpha (IL5Ra)

CDw125; CD125; IL5R-A; IL5-RA; HSIL5R3; IL5R; IL5R-A

ACTIVITY TEST

Interleukin 5 Receptor Alpha (IL5Ra) is a transmembrane glycoprotein encoded by the IL5RA gene, serving as the specific subunit of the IL-5 receptor complex. Expressed primarily on eosinophils, basophils, and B lymphocytes, it mediates the biological effects of IL-5, a key cytokine in allergic inflammation and eosinophil homeostasis. IL5Ra forms a heterodimer with the common beta chain (βc) to constitute the functional receptor, enabling signal transduction via JAK/STAT pathways. Dysregulation of IL5Ra is linked to eosinophilic disorders, asthma, and allergic diseases, making it a therapeutic target for anti-allergy and anti-inflammatory drugs. IL5 binds specifically to IL5Ra with high affinity, inducing receptor dimerization and activating downstream signaling cascades that promote eosinophil survival and activation. Briefly, IL5 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to IL5Ra-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti-IL5 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37℃, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 µL stop solution to the wells and read at 450/630nm immediately.Measured by its binding ability in a functional ELISA. When Recombinant IL5Ra is lmmobilized at 2 µg/mL(100 uLwell), the concentration of IL5 that produces 50% optimal bindingresponse is found to be approximately 0.0930 µg/mL.

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

GIVEAWAYS

INCREMENT SERVICES

Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
RPB510Hu01 Recombinant Interleukin 5 Receptor Alpha (IL5Ra) Positive Control; Immunogen; SDS-PAGE; WB.
EPB510Hu61 Eukaryotic Interleukin 5 Receptor Alpha (IL5Ra) Positive Control; Immunogen; SDS-PAGE; WB.
APB510Hu61 Active Interleukin 5 Receptor Alpha (IL5Ra) Cell culture; Activity Assays.
PAB510Hu01 Polyclonal Antibody to Interleukin 5 Receptor Alpha (IL5Ra) IHC
LAB510Hu71 Biotin-Linked Polyclonal Antibody to Interleukin 5 Receptor Alpha (IL5Ra) WB; IHC; ICC.
RAB510Hu21 Recombinant Antibody to Interleukin 5 Receptor Alpha (IL5Ra) WB; IF; ICC; IHC; IP; FCM.
MAB510Hu21 Monoclonal Antibody to Interleukin 5 Receptor Alpha (IL5Ra) WB
MAB510Hu23 Monoclonal Antibody to Interleukin 5 Receptor Alpha (IL5Ra) WB
MAB510Hu24 Monoclonal Antibody to Interleukin 5 Receptor Alpha (IL5Ra) WB
MAB510Hu22 Monoclonal Antibody to Interleukin 5 Receptor Alpha (IL5Ra) WB
SEB510Hu ELISA Kit for Interleukin 5 Receptor Alpha (IL5Ra) Enzyme-linked immunosorbent assay for Antigen Detection.
LMB510Hu Multiplex Assay Kit for Interleukin 5 Receptor Alpha (IL5Ra) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.