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Active B-Lymphocyte Activation Antigen B7-1 (LAB7-1)

CD80; CD28LG; CD28LG1; BB1; B7-1 Antigen; T-lymphocyte activation antigen CD80; CTLA-4 counter-receptor B7.1

ACTIVITY TEST

B-Lymphocyte Activation Antigen B7-1 (CD80) is a membrane receptor that is activated by the binding of CD28 or CTLA-4. The activated protein induces T-cell proliferation and cytokine production. This protein can act as a receptor for adenovirus subgroup B and may play a role in lupus neuropathy. A functional binding ELISA assay was conducted to detect the interaction of recombinant rat CD80 with recombinant human IL10. Briefly, recombinant rat CD80 were diluted serially in PBS, with 0.01% BSA (pH7.4). Duplicate samples of 100 ul were then transferred to IL10-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti-CD80 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 µL stop solution to the wells and read at 450 nm immediately. The binding activity of recombinant rat CD80 and recombinant human IL10 was shown in Figure 1, the EC50 for this effect is 0.09 ug/mL.

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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INCREMENT SERVICES

Magazine Citations
BioMed Research International Predictability of Urinary CD80 in the Relapse of Primary Nephrotic Syndrome pubmed:28951877
Catalog No. Related products for research use of Rattus norvegicus (Rat) Organism species Applications (RESEARCH USE ONLY!)
APB386Ra01 Active B-Lymphocyte Activation Antigen B7-1 (LAB7-1) Cell culture; Activity Assays.
RPB386Ra01 Recombinant B-Lymphocyte Activation Antigen B7-1 (LAB7-1) Positive Control; Immunogen; SDS-PAGE; WB.