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Active Pleiotrophin (PTN)

HARP; HBGF8; HBNF; NEGF1; Heparin Binding Growth Factor 8; Neurite Growth-Promoting Factor 1; Heparin Affin Regulatory Peptide; Heparin Binding Growth Associated Molecule

ACTIVITY TEST

Pleiotrophin (PTN) is a 136 amino acid secreted heparin-binding cytokine that signals diverse functions, including lineage-specific differentiation of glial progenitor cells, neurite outgrowth, and angiogenesis. A considerable amount of research has been carried out to understand the mechanisms by which PTN regulates these events. PTN has now been shown to bind a diverse collection of receptors including Syndecan 1 (SDC1). A functional binding ELISA assay was conducted to detect the interaction of recombinant human PTN and recombinant human SDC1. Briefly, PTN were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 µl were then transferred to SDC1-coated (2 ug/ml, 100 ul/well) microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with PTN pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of recombinant human PTN and recombinant human SDC1 was shown in Figure 1, the EC50 was 3.47 ug/ml.

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
Oncogene Suppression of lung adenocarcinoma through menin and polycomb gene-mediated repression of growth factor pleiotrophinMenin suppresses lung cancer through PTN Nature: 2009273
PLOS ONE The Effects of Pleiotrophin in Proliferative Diabetic Retinopathy Pubmed:25617851
Infect Immun.  Depletion of Phagocytic Cells during Nonlethal Plasmodium yoelii Infection Causes Severe Malaria Characterized by Acute Renal Failure in Mice. Pubmed:26755155
In Vivo Evaluation of Serum Biomarkers (FGF-2, HGF, MIF and PTN) in Patients With Testicular Germ Cell Cancer Pubmed: 31662522
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
APB309Hu01 Active Pleiotrophin (PTN) Cell culture; Activity Assays.
RPB309Hu02 Recombinant Pleiotrophin (PTN) Positive Control; Immunogen; SDS-PAGE; WB.
APB309Hu61 Active Pleiotrophin (PTN) Cell culture; Activity Assays.
RPB309Hu01 Recombinant Pleiotrophin (PTN) Positive Control; Immunogen; SDS-PAGE; WB.
EPB309Hu61 Eukaryotic Pleiotrophin (PTN) Positive Control; Immunogen; SDS-PAGE; WB.
PAB309Hu06 Polyclonal Antibody to Pleiotrophin (PTN) WB; IHC; ICC; IP.
PAB309Hu02 Polyclonal Antibody to Pleiotrophin (PTN) WB; IHC; ICC; IP.
PAB309Hu01 Polyclonal Antibody to Pleiotrophin (PTN) WB
LAB309Hu81 FITC-Linked Polyclonal Antibody to Pleiotrophin (PTN) WB; IHC; ICC; IF.
LAB309Hu71 Biotin-Linked Polyclonal Antibody to Pleiotrophin (PTN) WB; IHC; ICC.
MAB309Hu22 Monoclonal Antibody to Pleiotrophin (PTN) WB; IHC; ICC; IP.
SEB309Hu ELISA Kit for Pleiotrophin (PTN) Enzyme-linked immunosorbent assay for Antigen Detection.
SCB309Hu CLIA Kit for Pleiotrophin (PTN) Chemiluminescent immunoassay for Antigen Detection.
LMB309Hu Multiplex Assay Kit for Pleiotrophin (PTN) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.