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Active Milk Fat Globule EGF Factor 8 (MFGE8)

HMFG; MFGM; SED1; BA46; EDIL1; OAcGD3S; SPAG10; hP47; Medin; Lactadherin; Sperm Associated Antigen 10; Sperm Surface Protein hP47; Milk fat globule-EGF factor 8

ACTIVITY TEST

Milk Fat Globule EGF Factor 8 (MFGE8), also known as lactadherin, is a secreted glycoprotein containing EGF-like and coagulation factor C homology (C1/C2) domains. It mediates critical roles in apoptotic cell clearance (efferocytosis), tissue remodeling, angiogenesis, and immune regulation by binding integrin receptors (e.g., αvβ3/β5). MFGE8 facilitates phagocytosis of apoptotic cells via bridging phosphatidylserine on dying cells to phagocytes. It also modulates TGF-β activation and inflammatory responses. Expressed in macrophages, epithelial cells, and mammary glands, MFGE8 is implicated in atherosclerosis, autoimmune diseases, and cancer progression. Its dysfunction correlates with impaired tissue repair and aberrant immune activation.、Besides,EGF has been identified as an interactor of MFGE8, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant mouse MFGE8 and recombinant human EGF. Briefly,MFGE8 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to EGF-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti-MFGE8 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37℃, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 µL stop solution to the wells and read at 450/630nm immediately. The binding activity of recombinant mouse MFGE8 and recombinant human EGF was shown in Figure 1, the EC50 for this effect is 0.043ug/mL.

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
Fertility and Sterility Tumor necrosis factor α up-regulates endometrial milk fat globule-epidermal growth factor 8 protein production via nuclear factor κB activation, resulting in cell migration of epithelial cells. Pubmed: 24262600
U.S. Patent Application Biomarker for amyloid-b-related neurological disorders US20130302838
IUBMB Life. Elevated serum milk fat globule-epidermal growth factor 8 levels in type 2 diabetic patients are suppressed by overweight or obese status. pubmed:28067023
Journal of diabetes investigation Circulating milk fat globule-epidermal growth factor 8 levels are increased in pregnancy and gestational diabetes mellitus. pubmed:28035763
The Journal of Steroid Biochemistry and Molecular Biology Modifications of Western-type diet regarding protein, fat and sucrose levels as modulators of steroid metabolism and activity in liver pubmed:27471150
Journal of Steroid Biochemistry and Molecular Biology Depressive-like effect of prenatal exposure to DDT involves global DNA hypomethylation and impairment of GPER1/ESR1 protein levels but not ESR2 and AHR/ARNT signaling pubmed:28263910
Oncotarget Pantoprazole blocks the JAK2/STAT3 pathway to alleviate skeletal muscle wasting in cancer cachexia by inhibiting inflammatory response. pubmed:28489606
Molecular and Cellular Neuroscience TGF-β1 enhances phagocytic removal of neuron debris and neuronal survival by olfactory ensheathing cells via integrin/MFG-E8 signaling pathway 10.1016/j.mcn.2017.08.006
Amyloid Needle-shaped amyloid deposition in rat mammary gland: evidence of a novel amyloid fibril protein Pubmed: 31615282
clinical and translational medicine Milk fat globule EGF factor 8 restores mitochondrial function via integrin©\medicated activation of the FAK©\STAT3 signaling pathway in acute pancreatitis 33634976
Catalog No. Related products for research use of Mus musculus (Mouse) Organism species Applications (RESEARCH USE ONLY!)
RPB286Mu02 Recombinant Milk Fat Globule EGF Factor 8 (MFGE8) Positive Control; Immunogen; SDS-PAGE; WB.
APB286Mu01 Active Milk Fat Globule EGF Factor 8 (MFGE8) Cell culture; Activity Assays.
RPB286Mu01 Recombinant Milk Fat Globule EGF Factor 8 (MFGE8) Positive Control; Immunogen; SDS-PAGE; WB.
PAB286Mu01 Polyclonal Antibody to Milk Fat Globule EGF Factor 8 (MFGE8) WB
PAB286Mu02 Polyclonal Antibody to Milk Fat Globule EGF Factor 8 (MFGE8) WB; IHC; ICC; IP.
SEB286Mu ELISA Kit for Milk Fat Globule EGF Factor 8 (MFGE8) Enzyme-linked immunosorbent assay for Antigen Detection.
LMB286Mu Multiplex Assay Kit for Milk Fat Globule EGF Factor 8 (MFGE8) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.