≡

Active Taxilin Alpha (TXLNa)

IL14; TXLN; HMW-BCGF; High Molecular Weight B-Cell Growth Factor; Interleukin 14

ACTIVITY TEST

Taxilin Alpha (TXLNa) also known as interleukin-14 (IL-14) is a cytokine that controls the growth and proliferation of both normal and cancerous B cells. TXLNa induces B-cell proliferation, inhibits antibody secretion, and expands selected B-cell subgroups. This interleukin is produced mainly by T cells and certain malignant B cells. Besides, Phosphodiesterase 4D Interacting Protein (PDE4DIP) has been identified as an interactor of TXLNa, thus a binding ELISA assay was conducted to detect the interaction of recombinant mouse TXLNa and recombinant human PDE4DIP. Briefly, TXLNa were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100ul were then transferred to PDE4DIP-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-TXLNa pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution , wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of TXLNa and PDE4DIP was shown in Figure 1, the EC50 for this effect is 0.09ug/mL.

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

GIVEAWAYS

INCREMENT SERVICES

Catalog No. Related products for research use of Mus musculus (Mouse) Organism species Applications (RESEARCH USE ONLY!)
APB182Mu01 Active Taxilin Alpha (TXLNa) Cell culture; Activity Assays.
RPB182Mu01 Recombinant Taxilin Alpha (TXLNa) Positive Control; Immunogen; SDS-PAGE; WB.
PAB182Mu01 Polyclonal Antibody to Taxilin Alpha (TXLNa) WB; IHC; ICC; IP.
SEB182Mu ELISA Kit for Taxilin Alpha (TXLNa) Enzyme-linked immunosorbent assay for Antigen Detection.
LMB182Mu Multiplex Assay Kit for Taxilin Alpha (TXLNa) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.