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Figure. SDS-PAGE
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Active VGF Nerve Growth Factor Inducible (VGF)
NERP1; NERP2; Neuro-Endocrine Specific Protein VGF; Neuroendocrine Regulatory Peptide
- Product No.APB166Hu63
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Buffer FormulationPBS, pH7.4, containing 5% Trehalose.
- Traits Freeze-dried powder
- Purity> 90%
- Isoelectric Point4.4
- ApplicationsCell culture; Activity Assays.
- Downloadn/a
- UOM 10µg50µg 200µg 1mg 5mg
- FOB
US$ 348
US$ 869
US$ 1738
US$ 5214
US$ 13035
For more details, please contact local distributors!
ACTIVITY TEST
VGF (nerve growth factor inducible) is a secretory polypeptide precursor belonging to the granin/secretogranin family, selectively expressed in neuronal and neuroendocrine cells. It promotes cell proliferation, survival, and migration, while inhibiting apoptosis in neurons and multiple cancer cell types. VGF drives oncogenic signaling, enhances therapy resistance, and facilitates epithelial–mesenchymal transition. Its proteolytic products mediate neurogenesis, energy homeostasis, and tumor progression, positioning VGF as a key regulator in neural function and cancer pathogenesis.To test the effect of VGF on cell apoptosis, A549 cells were seeded into triplicate wells of 96-well plates and allowed to attach, replaced with various concentrations of recombinant human VGF. After incubated for 48 hours, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10 µl of CCK-8 solution was added to each well of the plate, then the absorbance at 450 nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37 ℃. Cell viability was assessed by CCK-8 (Cell Counting Kit-8 ) assay after incubation with recombinant human VGF for 48 hours. The result was shown in Figure 1. It was obvious that VGF significantly decreased cell viability of A549 cells.The ED50 of recombinant human VGF is 230 ng/mL.
USAGE
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
STORAGE
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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| Magazine | Citations |
| Journal of Neuroendocrinology | Proteomic analysis of cerebrospinal fluid from obese women with idiopathic intracranial hypertension: a new approach for identifying new candidates in the pathogenesis of obesity PubMed: 22296024 |
| J Psychiatr Res | Low VGF is associated with executive dysfunction in patients with major depressive disorder Pubmed:35738161 |