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Active Thioredoxin-interacting Protein (TXNIP)

TBP2; HHCPA78; THIF; VDUP1;Thioredoxin Binding Protein 2 ; Vitamin D3-Upregulated Protein 1; Upregulated By 1,25-Dihydroxyvitamin D-3

ACTIVITY TEST

Thioredoxin-interacting Protein (TXNIP), a multifunctional member of the α-arrestin family, is tightly linked to cellular metabolism, redox balance, and disease pathogenesis. Initially identified as a binding partner of thioredoxin, it regulates glucose/lipid metabolism via a glucose-responsive negative feedback loop and acts as a tumor suppressor by inhibiting cell proliferation. TXNIP expression is dysregulated in diverse diseases, including type 2 diabetes, cancer, and retinal or cardiac disorders, with its upregulation often exacerbating oxidative stress and inflammation. It localizes primarily in the cytoplasm and nucleus, participating in both cytoplasmic signaling and nuclear gene regulation. TXNIP directly binds to NLRP3 to activate the inflammasome, triggering inflammatory cytokine release and cell pyroptosis.Thus a functional ELISA assay was conducted to detect the interaction of recombinant mouse TXNIP and recombinant human NLRP3. Briefly, TXNIP was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to NLRP3-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti-TXNIP pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37℃, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 µL stop solution to the wells and read at 450/630nm immediately. The binding activity of recombinant mouse TXNIP and recombinant human NLRP3 was shown in Figure 1, the EC50 for this effect is 0.01193µg/mL.

USAGE

Reconstitute in ddH2O to a concentration of 0.1-0.2 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
Human Reproduction The roles of thioredoxin and thioredoxin-binding protein-2 in endometriosis OxfordJournals: source
Reproduction Alteration in the intrafollicular thiol–redox system in infertile women with endometriosis Pubmed:25376627
Catalog No. Related products for research use of Mus musculus (Mouse) Organism species Applications (RESEARCH USE ONLY!)
RPB162Mu02 Recombinant Thioredoxin-interacting Protein (TXNIP) Positive Control; Immunogen; SDS-PAGE; WB.
APB162Mu01 Active Thioredoxin-interacting Protein (TXNIP) Cell culture; Activity Assays.
RPB162Mu01 Recombinant Thioredoxin-interacting Protein (TXNIP) Positive Control; Immunogen; SDS-PAGE; WB.
PAB162Mu01 Polyclonal Antibody to Thioredoxin-interacting Protein (TXNIP) WB; IHC
PAB162Mu02 Polyclonal Antibody to Thioredoxin-interacting Protein (TXNIP) WB; IHC; ICC; IP.
SEB162Mu ELISA Kit for Thioredoxin-interacting Protein (TXNIP) Enzyme-linked immunosorbent assay for Antigen Detection.
LMB162Mu Multiplex Assay Kit for Thioredoxin-interacting Protein (TXNIP) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.