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Active Integrin Alpha X (CD11c)

ITGaX; CD11-C; ITG-AX; ITGA-X; Leu M5; Complement Component 3 Receptor 4 Subunit; CD11 antigen-like family member C; Leukocyte adhesion glycoprotein p150,95 alpha chain

ACTIVITY TEST

Integrin Alpha X (CD11c) is a member of the leukointegrin family, it is an approximately 150 kDa type I transmembrane glycoprotein that heterodimerizes with Integrin beta 2. One of the earliest described properties of CD11c is its ability to mediate phagocytosis of iC3b-opsonized particles. Accordingly, CD11c is known as complement receptor 4. CD11c has also been implicated in phagocytosis of latex beads and bacteria in the absence of complement. CD11c binds a diverse array of ligands such as cell adhesion molecules and Interleukin 13 (IL13). Thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human CD11c and recombinant bovine IL13. Briefly, CD11c was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to IL13-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti-CD11c pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37℃, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 µl stop solution to the wells and read at 450/630 nm immediately. The binding activity of recombinant human CD11c and recombinant bovine IL13 was shown in Figure 1, the EC50 for this effect is 0.28 ug/mL.

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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