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Active Arginase (ARG)

ARG1; Arginase I; Liver Arginase

ACTIVITY TEST

Arginase (Arg) is an enzyme that catalyzes the degradation of arginine to produce urea and omithine, which is crucial in the urea cycle. In most mammals, two isozymes of this enzyme exist; the first, Arginase I, functions in the urea cycle, and is located primarily in the cytoplasm of the liver. The second isozyme, Arginase II, has been implicated in the regulation of the arginine/ornithine concentrations in the cell. Besides, Ubiquitin Carboxyl Terminal Hydrolase L5 (UCHL5) has been identified as an interactor of Arg, thus a binding ELISA assay was conducted to detect the interaction of recombinant mouse Arg and recombinant mouse UCHL5. Briefly, Arg were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to UCHL5-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-Arg pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of of Arg and UCHL5 was shown in Figure 1, and this effect was in a dose dependent manner.

USAGE

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
PLoS One Phenobarbital Induces Alterations in the Proteome of Hepatocytes and Mesenchymal Cells of Rat Livers Pubmed: 24204595
J Cereb Blood Flow Metab Arginase I release from activated neutrophils induces peripheral immunosuppression in a murine model of stroke PubMed: 25966956
Cancer Medicine Circulating CD14+HLA-DR-/low myeloid-derived suppressor cells in leukemia patients with allogeneic hematopoietic stem cell transplantation: novel clinical potential strategies for the prevention and cellular therapy of graft-versus-host disease Pubmed:27109254
Cell Mol Immunol  Toxoplasma gondii GRA15II effector-induced M1 cells ameliorate liver fibrosis in mice infected with Schistosomiasis japonica Pubmed:27157496
Potent but transient immunosuppression of T-cells is a general feature of erythroid progenitor cells
Commun Biol Potent but transient immunosuppression of T-cells is a general feature of CD71+ erythroid cells 34893694
Catalog No. Related products for research use of Mus musculus (Mouse) Organism species Applications (RESEARCH USE ONLY!)
RPB120Mu02 Recombinant Arginase (ARG) Positive Control; Immunogen; SDS-PAGE; WB.
RPB120Mu01 Recombinant Arginase (ARG) Positive Control; Immunogen; SDS-PAGE; WB.
APB120Mu01 Active Arginase (ARG) Cell culture; Activity Assays.
PAB120Mu01 Polyclonal Antibody to Arginase (ARG) WB; IHC; ICC; IP.
PAB120Mu02 Polyclonal Antibody to Arginase (ARG) WB; IHC; ICC; IP.
LAB120Mu81 FITC-Linked Polyclonal Antibody to Arginase (ARG) WB; IHC; ICC; IF.
LAB120Mu71 Biotin-Linked Polyclonal Antibody to Arginase (ARG) WB; IHC; ICC.
SEB120Mu ELISA Kit for Arginase (ARG) Enzyme-linked immunosorbent assay for Antigen Detection.
MEB120Mu Mini Samples ELISA Kit for Arginase (ARG) Enzyme-linked immunosorbent assay for Antigen Detection.
LMB120Mu Multiplex Assay Kit for Arginase (ARG) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
KSB120Mu01 ELISA Kit DIY Materials for Arginase (ARG) Main materials for "Do It (ELISA Kit) Yourself".