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Active Bone Sialoprotein (BSP)

IBSP; Bsp; BspII; SpII; Integrin-Binding Bone Sialoprotein; Cell-binding sialoprotein; Bone sialoprotein 2

ACTIVITY TEST

Bone Sialoprotein (BSP) is a highly glycosylated and phosphorylated non-collagenous protein that plays a pivotal role in mineralized tissues, particularly in bone and dentin. As a member of the small integrin-binding ligand N-linked glycoprotein (SIBLING) family, it is primarily synthesized by osteoblasts, odontoblasts, and chondrocytes, and is prominently localized in the extracellular matrix of mineralizing tissues. Additionally, it also has the function of cell adhesion. Therefore, we measure the activity of recombinant rat BSP by the ability of the immobilized protein to support the adhesion of MDA-MB-231 cells. When 5 x 104 cells/well are added to different concentrations of recombinant rat BSP coated plates, cells will adhere after 2 hour incubation at 37 ℃. The adhesion of MDA-MB-231 cells after 2 hour incubation at 37 ℃ observed by inverted microscope was shown in Figure 1. Cell adherent was in a dose dependent manner, the result was shown in Figure 2, the EC50 was 0.48 μg/ml.

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
FEBS Letters Low-intensity pulsed ultrasound-induced ATP increases bone formation via the P2X7 receptor in osteoblast-like MC3T3-E1 cells Pubmed:25542352
Journal of Dental Research Adiponectin Promotes Human Jaw Bone Marrow Stem Cell Osteogenesis Pubmed:26961489
circulation journal Stenotic Bicuspid and Tricuspid Aortic Valves　- Micro-Computed Tomography and Biological Indices of Calcification. pubmed:28344201
Catalog No. Related products for research use of Rattus norvegicus (Rat) Organism species Applications (RESEARCH USE ONLY!)
APB092Ra61 Active Bone Sialoprotein (BSP) Cell culture; Activity Assays.