Active Alkaline Phosphatase, Tissue-nonspecific (ALPL)

BALP; BSAP; HOPS; AP-TNAP; TNSALP; Bone Alkaline Phosphatase; Alkaline Phosphatase,Tissue-Nonspecific Isozyme; Alkaline Phosphatase, Liver/Bone/Kidney

ACTIVITY TEST

Four distinct genes encode alkaline phosphatases (APs) in humans. The ALPL gene encodes the liver/bone/kidney isozyme, also known as the tissuenonspecific AP (TNAP). In comparison, ALPI, ALPP and ALPPL2 encode intestinal, placental and placental-like or germ cell APs, respectively. The serum levels of human APs are useful tumor markers. There are many mutations in the ALPL gene, leading to different forms of hypophosphatasia, characterized by poorly mineralized cartilage and bones. The native ALPL is a glycosylated homodimer attached to the membrane through a GPI-anchor. The activity assay of recombinant mouse ALPL was measured by its ability to cleave a peptide substrate, 4-Methumbelliferly phosphate. The reaction was performed in 50 mM Tris, 1 mM MgCl2, pH 9.0 ( assay buffer), ainitiated by addition 50 μl of 0.3 ug/ml ALPL (diluted by assay buffer) to 50 µL of 50 uM substrate. Read at excitation and emission wavelengths of 365 nm and 445 nm (top read), respectively, in kinetic mode for 5 minutes. The specific activity of recombinant mouse ALPL is >11000 pmol/min/µg.

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
Journal of Materials Science: Materials in Medicine Osteopenic bone cell response to strontium-substituted hydroxyapatite SpringerLink: n8233337w171v451
Osteoporosis International Dextromethorphan inhibits osteoclast differentiation by suppressing RANKL-induced nuclear factor-κB activation PubMed: 23400250
Journal of Applied Toxicology Chronic exposure to low concentrations of strontium 90 affects bone physiology but not the hematopoietic system in mice Wiley: Source
Acta Pharmacologica Sinica The antidepressant bupropion exerts alleviating properties in an ovariectomized osteoporotic rat model Pubmed:25544359
Biomedical Materials Enhanced differentiation of osteoblastic cells on novel chitosan/β-1, 3-glucan/bioceramic scaffolds for bone tissue regeneration Pubmed:25586067
Pharmacology Effect of Mirtazapine on Rat Bone Tissue after Orchidectomy PubMed: 25871861
J Biomed Mater Res A. New method for the fabrication of highly osteoconductive β-1,3-glucan/HA scaffold for bone tissue engineering: Structural, mechanical, and biological characterization. Pubmed:27239050
J Bone Miner Metab MiR-142-5p promotes bone repair by maintaining osteoblast activity Pubmed:27085967
Journal of Biomedical Materials Research New method for the fabrication of highly osteoconductive β‐1, 3‐glucan/HA scaffold for bone tissue engineering: Structural, mechanical, and biological … doi:10.1002
Journal of Bone and Mineral Metabolism MiR‑142‑5p promotes bone repair by maintaining osteoblast activity pubmed:27085967
Scientific Reports Hydrolysis of Extracellular Pyrophosphate increases in post-hemodialysis plasma Pubmed:30038263
International Journal of Molecular Sciences  Suppression Effect of Astaxanthin on Osteoclast Formation In Vitro and Bone Loss In Vivo Pubmed:29562730
Pharmacology Effects of Amlodipine on Bone Metabolism in Orchidectomised Spontaneously Hypertensive Rats Pubmed:29898457
Experimental and Therapeutic Medicine Combination therapy with BMP‑2 and psoralen enhances fracture healing in ovariectomized mice 10.3892:etm.2018.6353
American Journal of Translational Research Long non-coding RNA SNHG7 promotes the fracture repair through negative modulation of miR-9
The effect of low temperature atmospheric nitrogen plasma on MC3T3-E1 preosteoblast proliferation and differentiation in vitro
International Journal of Biomaterials Bovine Hydroxyapatite-Based Bone Scaffold with Gentamicin Accelerates Vascularization and Remodeling of Bone Defect 34104195
Catalog No. Related products for research use of Mus musculus (Mouse) Organism species Applications (RESEARCH USE ONLY!)
APB091Mu61 Active Alkaline Phosphatase, Tissue-nonspecific (ALPL) Cell culture; Activity Assays.
RPB091Mu02 Recombinant Alkaline Phosphatase, Tissue-nonspecific (ALPL) Positive Control; Immunogen; SDS-PAGE; WB.
EPB091Mu61 Eukaryotic Alkaline Phosphatase, Tissue-nonspecific (ALPL) Positive Control; Immunogen; SDS-PAGE; WB.
RPB091Mu01 Recombinant Alkaline Phosphatase, Tissue-nonspecific (ALPL) Positive Control; Immunogen; SDS-PAGE; WB.
PAB091Mu02 Polyclonal Antibody to Alkaline Phosphatase, Tissue-nonspecific (ALPL) WB; IHC; ICC; IP.
PAB091Mu01 Polyclonal Antibody to Alkaline Phosphatase, Tissue-nonspecific (ALPL) WB; IHC; ICC; IP.
LAB091Mu81 FITC-Linked Polyclonal Antibody to Alkaline Phosphatase, Tissue-nonspecific (ALPL) WB; IHC; ICC; IF.
LAB091Mu71 Biotin-Linked Polyclonal Antibody to Alkaline Phosphatase, Tissue-nonspecific (ALPL) WB; IHC; ICC.
SEB091Mu ELISA Kit for Alkaline Phosphatase, Tissue-nonspecific (ALPL) Enzyme-linked immunosorbent assay for Antigen Detection.
LMB091Mu Multiplex Assay Kit for Alkaline Phosphatase, Tissue-nonspecific (ALPL) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.