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Active Glutathione S Transferase Pi (GSTp)

GSTP1; GST-P1; DFN7; FAEES3; GST3

ACTIVITY TEST

Glutathione S Transferase Pi (GSTp) is a key member of the GST enzyme family, widely expressed in mammalian tissues like the liver, kidneys, and lungs. It primarily functions in cellular detoxification, catalyzing the conjugation of glutathione to electrophilic xenobiotics and endogenous reactive species, facilitating their excretion. Beyond detoxification, GSTp also regulates cellular signaling and stress responses—for instance, it modulates oxidative stress by scavenging free radicals and interacts with signaling molecules to influence cell survival. Structurally, it exists as a homodimer, with each subunit containing a glutathione-binding site and a substrate-binding site, enabling its enzymatic and regulatory roles. GSTp specifically binds to Mitogen Activated Protein Kinase 8 (MAPK8), inhibiting MAPK8’s kinase activity to regulate stress and apoptotic pathways.To detect the activity of recombinant GSTp a functional ELISA assay was performed to evaluate the interaction between recombinant pig GSTp and recombinant human MAPK8.Briefly, GSTp was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to MAPK8-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti-GSTp pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37℃, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 µL stop solution to the wells and read at 450/630nm immediately. The binding activity of recombinant pig GSTp and recombinant human MAPK8 was shown in Figure 1, the EC50 for this effect is 0.31µg/mL.

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
International Journal of Medical Sciences Glutathione S-Transferase P1 Correlated with Oxidative Stress in Hepatocellular Carcinoma PubMed: PMC3619117
Mol Neurobiol Human Glutathione S-Transferase Enzyme Gene Polymorphisms and Their Association With Neurocysticercosis Pubmed:27021019
Journal of Biomaterials and Tissue Engineering 3D Printing of Differentiated Bone Marrow Mesenchymal Cells as a New Method for Liver Tissue Engineering art00007
Neuroscience Bulletin Comprehensive proteomic profiling of patients' tears identifies potential biomarkers for the traumatic vegetative state Pubmed:30019218
Arabian Journal of Chemistry Attenuating effect of Indole-3-Carbinol on gold nanoparticle induced hepatotoxicity in rats
International Journal of Nature and Life Sciences The Role of Glutathione S-Transferases in Pleomorphic Adenomas of the Salivary Glands
Catalog No. Related products for research use of Sus scrofa; Porcine (Pig) Organism species Applications (RESEARCH USE ONLY!)
APB090Po01 Active Glutathione S Transferase Pi (GSTp) Cell culture; Activity Assays.
RPB090Po01 Recombinant Glutathione S Transferase Pi (GSTp) Positive Control; Immunogen; SDS-PAGE; WB.
PAB090Po01 Polyclonal Antibody to Glutathione S Transferase Pi (GSTp) WB; IHC
FAB090Po01 Anti-Glutathione S Transferase Pi (GSTp) Polyclonal Antibody FCM
MAB090Po21 Monoclonal Antibody to Glutathione S Transferase Pi (GSTp) WB; IHC; ICC; IP.
SEB090Po ELISA Kit for Glutathione S Transferase Pi (GSTp) Enzyme-linked immunosorbent assay for Antigen Detection.
LMB090Po Multiplex Assay Kit for Glutathione S Transferase Pi (GSTp) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.