Active Superoxide Dismutase 2, Mitochondrial (SOD2)
IPO-B; MNSOD; Mn-SOD
- Product No.APB083Ra01
- Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
- Buffer FormulationPBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
- Traits Freeze-dried powder
- Purity> 95%
- Isoelectric Point7.9
- ApplicationsCell culture; Activity Assays.
- DownloadInstruction Manual
- UOM 10µg50µg 200µg 1mg 5mg
- FOB
US$ 208
For more details, please contact local distributors! US$ 520 US$ 1040 US$ 3120 US$ 7800
ACTIVITY TEST
![](/upload/product/pictures/APB083Ra01-202401231708145932.jpg)
Extracellular superoxide dismutase [Cu-Zn] is an enzyme that in humans is encoded by the SOD2 gene. This gene encodes a member of the superoxide dismutase (SOD) protein family. SODs are antioxidant enzymes that catalyze the dismutation of two superoxide radicals into hydrogen peroxide and oxygen. Acroding to the report, in a weakly alkaline buffer solution (pH=8.2) with N-tris(hydroxymethyl)amino methane-HCL, pyrogallol can occur autoxidation in the air, then SOD can inhibit this reaction. Thus, we use this way to measued the activity of recombinant rat SOD2. The reaction was performed in adding 8 μl 5 mmol/L pyrogallol to 200 μl 50mmol/L Tris-HCl, rapidly mixing at 25 ℃, then read at 325 nm (using 50mmol/L Tris-HCl as blank control) in kinetic mode for 3 minutes using a microplate reader controlling the pyrogallol autoxidation rate at 0.70 OD/min. Different concentrations of recombinant rat SOD2 were added into 200 μl 50 mmol/L Tris-HCl, incubated for 20 min at 25 ℃, then adding 8 μl 5 mmol/L pyrogallol to each well, rapidly mixing and read at 325 nm in kinetic mode for 3 minutes. Under these conditions, the enzyme amount of 50% inhibition of pyrogallol autooxidation per minute is defined as a unit. The specific activity of recombinant rat SOD2 is 390 U/mg.
USAGE
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
STORAGE
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
GIVEAWAYS
INCREMENT SERVICES
-
BCA Protein Quantification Kit
-
Molecular Mass Marker for Protein
-
Monoclonal Antibody Customized Service
-
Polyclonal Antibody Customized Service
-
Protein Activity Test Experiment Service
-
Electrophoretic Mobility Shift Assay (EMSA) Experiment Service
-
Buffer
-
Lentivirus Packaging Experiment Service
-
Adenovirus Packaging Experiment Service
-
Real Time PCR Experimental Service
-
Spike RBD Protein (S-RBD)
-
Protein G
-
Protein A
Magazine | Citations |
Oral Disease | Antioxidant profile of salivary glands in high fat diet-induced insulin resistance rats Pubmed: 24106991 |
AGE | Consequences of age on ischemic wound healing in rats: altered antioxidant activity and delayed wound closure Pubmed:24443098 |
Anticancer Research | Determination of Gene Expression and Serum Levels of MnSOD and GPX1 in Colorectal Cancer Pubmed:25550558 |
Arch Oral Biol | Antioxidant profile, carbonyl and lipid oxidation markers in the parotid and submandibular glands of rats in different periods of streptozotocin induced diabetes PubMed: 26143097 |
J Oral Pathol Med | Impact of morbid obesity and bariatric surgery on antioxidant/oxidant balance of the unstimulated and stimulated human saliva PubMed: 26608886 |
Free Radic Biol Med. | DDAH1 deficiency promotes intracellular oxidative stress and cell apoptosis via a miR-21-dependent pathway in mouse embryonic fibroblasts. Pubmed:26806551 |
Basic Research in Cardiology | Cardiomyocyte dimethylarginine dimethylaminohydrolase1 attenuates left-ventricular remodeling after acute myocardial infarction: involvement in oxidative stress and … Pubmed:29892894 |
Journal of Pharmacological Sciences | Improved endogenous epoxyeicosatrienoic acid production mends heart function via increased PGC 1α-mitochondrial functions in metabolic syndrome Pubmed: 30342783 |
Advances in Medical Sciences | Selected elements of extracellular matrix of the skin in diabetes and insulin resistance Pubmed: 31146169 |
PHARMACOLOGICAL RESEARCH | A Machine Learning-driven Study Indicates Emodin Improves Cardiac Hypertrophy by Modulation of Mitochondrial SIRT3 Signaling Pubmed: 32135248 |
Biomolecules | Arachidonic Acid as an Early Indicator of Inflammation during Non-Alcoholic Fatty Liver Disease Development Pubmed: 32751983 |
Nutrients | Attenuation of Oxidative Stress and Inflammatory Response by Chronic Cannabidiol Administration Is Associated with Improved n-6/n-3 PUFA Ratio in the White and?¡ 34064937 |
Pharmaceuticals | Serum Total SOD Activity and SOD1/2 Concentrations in Predicting All-Cause Mortality in Lung Cancer Patients 34832849 |
Biochimica et Biophysica Acta-Molecular Basis of Disease | α-Lipoic acid ameliorates inflammation state and oxidative stress by reducing the content of bioactive lipid derivatives in the left ventricle of rats fed a high-fat diet Pubmed:35569738 |