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Active Integrin Beta 1 (ITGb1)

CD29; MSK12; ITG-B1; FNRB; GPIIA; MDF2; MSK12; VLAB; Fibronectin Receptor Beta Polypeptide; Antigen CD29 Includes MDF2; Glycoprotein IIa; VLA-4 subunit beta

ACTIVITY TEST

Integrin Beta 1 (ITGb1), also known as CD29, is a fundamental subunit of the integrin family of adhesion receptors. It is ubiquitously expressed on cell surfaces and can heterodimerize with various alpha subunits to form distinct integrins. These heterodimers are crucial for mediating bidirectional signaling between the cell and its extracellular matrix (ECM), influencing essential processes such as cell adhesion, migration, proliferation, and survival. ITGb1 binds to ECM proteins like fibronectin and laminin, playing a vital role in embryonic development, tissue repair, and immune responses. Specifically, ITGb1 non-covalently associates with Integrin Alpha 3 (ITGa3) to form a functional receptor for laminin-5 in epithelial cells.To detect the activity of recombinant ITGb1, a functional ELISA assay was performed to evaluate the interaction between recombinant rat ITGb1 and recombinant mouse ITGa3. Briefly, biotin-linked ITGb1 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100µl were then transferred to ITGa3-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST 3 times and incubation with Streptavidin-HRP for 30min, then wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µl stop solution to the wells and read at 450nm immediately. The binding activity of recombinant rat ITGb1 and recombinant mouse ITGa3 was shown in Figure 1, the EC50 for this effect is 0.73µg/mL.

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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RPB042Ra01 Recombinant Integrin Beta 1 (ITGb1) Positive Control; Immunogen; SDS-PAGE; WB.
APB042Ra01 Active Integrin Beta 1 (ITGb1) Cell culture; Activity Assays.
RPB042Ra02 Recombinant Integrin Beta 1 (ITGb1) Positive Control; Immunogen; SDS-PAGE; WB.
RPB042Ra03 Recombinant Integrin Beta 1 (ITGb1) Positive Control; Immunogen; SDS-PAGE; WB.
RPB042Ra04 Recombinant Integrin Beta 1 (ITGb1) Positive Control; Immunogen; SDS-PAGE; WB.