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Active Renin (REN)

angiotensinogenase

ACTIVITY TEST

Renin is an enzyme that plays a crucial role in the regulation of blood pressure and electrolyte balance by activating the renin-angiotensin-aldosterone system (RAAS). It is secreted by the kidneys in response to low blood pressure or low sodium levels.The activity of recombinant human REN is measured by its ability to cleave a fluorogenic peptide substrate Arg-Glu(EDANS)-Ile-His-Pro-Phe-His-Pro-Phe-His-Leu-Val-Ile-His-Thr-Lys(dabcyl)-Arg
in the assay buffer 50 mM Sodium Acetate, 150 mM NaCl, 2 mM EDTA, pH 5.0 .Recombinant human REN was diluted to 200ug/ml in the activation buffer 5 mM Tris, 15 mM NaCl, 1 mM CaCl2, 0.005% Brij-35, pH 7.5, then activated by Trypsin in a final concentration of 4ug/ml incubated at 37 °C for 1 hours. Stop activation with PMSF at 1 mM and incubate at room temperature for 30 min.The activated rhREN is diluted toto different concentrations in assay buffer. Loading into a black well plate 50 µL rhREN and start the reaction by adding 50 µL of 20 µM substrate, with a substrate blank containing 50 µL assay buffer, 50 µL substrate, and no rhREN.Then read at excitiation and emission wavelengths of350 nm and 490 nm, respectively, in kinetic mode for 5 minutes. The specific activity of rhREN is >13 pmol/min/µg

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
Hypertension Prenatal overexposure to glucocorticoids programs renal 11β-hydroxysteroid dehydrogenase type 2 expression and salt-sensitive hypertension in the rat PubMed: 21045727
The Journal of Physiology Recombinant human erythropoietin in humans down-regulates proximal renal tubular reabsorption and causes a fall in glomerular filtration rate Physoc: 194241
The Journal of Physiology Erythropoietin down‐regulates proximal renal tubular reabsorption and causes a fall in glomerular filtration rate in humans PubMed: 20724370
Pathobiology Anti-Angiotensin and Hypoglycemic Treatments Suppress Liver Metastasis of Colon Cancer Cells Karger: 000330169
Journal of Cardiovascular Pharmacology The Angiotensin Receptor Blocker, Azilsartan Medoxomil (TAK-491), Suppresses Vascular Wall Expression of Plasminogen Activator Inhibitor Type-I Protein Potentially Facilitating the Stabilization of Atherosclerotic Plaques LWW: 08000
Kidney International Multiple and large simple renal cysts are associated with prehypertension and hypertension. Pubmed: 23389415
Human Physiology Estimation of the parameters of the renin-angiotensin-aldosterone system, water-electrolyte metabolism, and endothelial function in newborns of women with chronic hypertension Springer:Source
Journal of Pharmacological Sciences Organ-Specific Activation of the Gastric Branch of the Efferent Vagus Nerve by Ghrelin in Urethane-Anesthetized Rats Pubmed:24366191
Liver International VSL#3 probiotic treatment decreases bacterial translocation in rats with carbon tetrachloride-induced cirrhosis Pubmed:24750552
Gynecological Endocrinology Immunological and hemostasiological disorders in women with ovarian hyperstimulation syndrome Pubmed:25200828
Oxidative Medicine and Cellular Longevity Age-Associated Changes in the Vascular Renin-Angiotensin System in Mice Pubmed:27200147
PLoS One Ablation of Potassium-Chloride Cotransporter Type 3 (Kcc3) in Mouse Causes Multiple Cardiovascular Defects and Isosmotic Polyuria Pubmed:27166674
PLOS ONE Reduced Expression of the Extracellular Calcium-Sensing Receptor (CaSR) Is Associated withActivation of the Renin-Angiotensin System (RAS) to Promote Vascular Remodeling in the Pathogenesis of Essential Hypertension. pubmed:27391973
Heart Lung Circ. Efficacy and Safety of Renal Sympathetic Denervation on Dogs with Pressure Overload-Induced Heart Failure. pubmed:27555054
European Journal of Pharmaceutical Sciences Sacubitril/valsartan (LCZ696) Significantly Reduces Aldosterone and Increases cGMP Circulating Levels in a Canine Model of RAAS Activation Doi: 10.1101/435560
Nutrition Gut Dysbiosis Contribute to High Fructose Induced Salt Sensitive Hypertension In Sprague-Dawley Rats
ACTA PHARMACOLOGICA SINICA The vascular endothelial growth factor trap aflibercept induces vascular dysfunction and hypertension via attenuation of eNOS/NO signaling in mice 33303990
Hypertens Res Association of Ang-(1¨C7) and des-Arg 9 BK as new biomarkers of obesity and cardiometabolic risk factors in adolescents 33568792
Phosphorylation of β1-integrin in juxtaglomerular cells helps control blood pressure during the progression of diabetic nephropathy
Bedeutung des intrarenalen Renin-Angiotensin-Systems f¨¹r die Entwicklung der chronischen Nierenkrankheit
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
APA889Hu61 Active Renin (REN) Cell culture; Activity Assays.
EPA889Hu61 Eukaryotic Renin (REN) Positive Control; Immunogen; SDS-PAGE; WB.
RPA889Hu01 Recombinant Renin (REN) Positive Control; Immunogen; SDS-PAGE; WB.
PAA889Hu01 Polyclonal Antibody to Renin (REN) WB; IHC; ICC; IP.
MAA889Hu23 Monoclonal Antibody to Renin (REN) WB; IHC; ICC; IP.
MAA889Hu21 Monoclonal Antibody to Renin (REN) WB; IHC; ICC; IP.
MAA889Hu25 Monoclonal Antibody to Renin (REN) WB; IHC; ICC; IP.
MAA889Hu24 Monoclonal Antibody to Renin (REN) WB; IHC; ICC; IP.
MAA889Hu22 Monoclonal Antibody to Renin (REN) WB; IHC; ICC; IP.
SEA889Hu ELISA Kit for Renin (REN) Enzyme-linked immunosorbent assay for Antigen Detection.
SCA889Hu CLIA Kit for Renin (REN) Chemiluminescent immunoassay for Antigen Detection.
LMA889Hu Multiplex Assay Kit for Renin (REN) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.