Active Receptor Activator Of Nuclear Factor Kappa B Ligand (RANkL)
CD254; TNFSF11; ODF; OPGL; TRANCE; HRANKL2; SOdf; Tumor Necrosis Factor(ligand)superfamily Member 11; TNF-related activation-induced cytokine
- Product No.APA855Mu01
- Organism SpeciesMus musculus (Mouse) Same name, Different species.
- Buffer Formulation20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
- Traits Freeze-dried powder
- Purity> 90%
- Isoelectric Point7.1
- ApplicationsCell culture; Activity Assays.
- DownloadInstruction Manual
- UOM 10µg50µg 200µg 1mg 5mg
- FOB
US$ 310
For more details, please contact local distributors! US$ 774 US$ 1548 US$ 4644 US$ 11610
ACTIVITY TEST
Figure. The binding activity of RANKL with PPARg.
Receptor activator of nuclear factor kappa-B ligand (RANKL), also known as tumor necrosis factor ligand superfamily member 11 (TNFSF11), TNF-related activation-induced cytokine (TRANCE), osteoprotegerin ligand (OPGL), and osteoclast differentiation factor (ODF), is a protein that in humans is encoded by the TNFSF11 gene. RANKL is a member of the tumor necrosis factor (TNF) cytokine family, it binds to RANK on cells of the myeloid lineage and functions as a key factor for osteoclast differentiation and activation. It may also bind to osteoprotegerin, a protein secreted mainly by cells of the osteoblast lineage which is a potent inhibitor of osteoclast formation by preventing binding of RANKL to RANK. RANKL also has a function in the immune system, where it is expressed by T helper cells and is thought to be involved in dendritic cell maturation. This protein was shown to be a dendritic cell survival factor and is involved in the regulation of T cell-dependent immune response. Besides, Peroxisome Proliferator Activated Receptor Gamma (PPARg) has been identified as an interactor of RANKL, thus a binding ELISA assay was conducted to detect the interaction of recombinant mouse RANKL and recombinant mouse PPARg. Briefly, RANKL were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then
transferred to PPARg-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-RANKL pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of RANKLand PPARg was shown in Figure 1, and this effect was in a dose dependent manner.
USAGE
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
STORAGE
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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INCREMENT SERVICES
- BCA Protein Quantification Kit
- Molecular Mass Marker for Protein
- Monoclonal Antibody Customized Service
- Polyclonal Antibody Customized Service
- Protein Activity Test Experiment Service
- Electrophoretic Mobility Shift Assay (EMSA) Experiment Service
- Buffer
- Lentivirus Packaging Experiment Service
- Adenovirus Packaging Experiment Service
- Real Time PCR Experimental Service
- Spike RBD Protein (S-RBD)
- Protein G
- Protein A
Magazine | Citations |
Archives of Oral Biology | Compressive stress induced the up-regulation of M-CSF, RANKL, TNF-α expression and the down-regulation of OPG expression in PDL cells via the integrin-FAK pathway ScienceDirect: S0003996912003998 |
PLOS One | Adipokines in Psoriatic Arthritis Patients: The Correlations with Osteoclast Precursors and Bone Erosions PubMed: PMC3483160 |
Arthritis Research&Therapy | Regulatory effect of calcineurin inhibitor, tacrolimus, on IL-6/sIL-6R-mediated RANKL expression through JAK2-STAT3-SOCS3 signaling pathway in fibroblast-like synoviocytes PubMed: PMC3672788 |
International Journal of Oral and Maxillofacial Surgery | The effects of chronic zoledronate usage on the jaw and long bones evaluated using RANKL and osteoprotegerin levels in an animal model Pubmed: 23522850 |
AGE | Estrogen deficiency does not decrease the in vitro osteogenic potential of rat adipose-derived mesenchymal stem cells Pubmed: 24687841 |
Food Science & Nutrition | Effects and mechanisms of 8-prenylnaringenin on osteoblast MC3T3-E1 and osteoclast-like cells RAW264.7 Onlinelibrary: fsn3.109 |
Food Sci Nutr. | Effects and mechanisms of 8‐prenylnaringenin on osteoblast MC3T3‐E1 and osteoclast‐like cells RAW264. 7 Pubmed:Pmc4221832 |
Journal of Investigative Surgery | Effect of Nicotine on RANKL and OPG and Bone Mineral Density Pubmed:24830656 |
Int J Mol Med. | Effects of icariin on the regulation of the OPG?RANKL?RANK system are mediated through the MAPK pathways in IL?1β?stimulated human SW1353 chondrosarcoma … Pubmed:25270538 |
Clinical and Experimental Pharmacology and Physiology | Carvedilol promotes neurological function, reduces bone loss and attenuates cell damage after acute spinal cord injury in rats Pubmed:25424914 |
Int J Clin Exp Pathol | A novel model of bisphosphonate-related osteonecrosis of the jaw in rats PubMed: 26191212 |
oncotarget | MicroRNA-106b inhibits osteoclastogenesis and osteolysis by targeting RANKL in giant cell tumor of bone PubMed: 26053181 |
European Journal of Clinical Investigation | Serum osteopontin levels are upregulated and predict disability after an ischaemic stroke PubMed: 25845543 |
Scienze Biomediche | Effetti del laser a bassa dose su cellule osteoblastiche INCERTIPARENTI_SERENA_TESI.pdf |
Nutrition. | Noni leaf and black tea enhance bone regeneration in estrogen-deficient rats. pubmed:27908549 |
PLoS One. | Extracorporeal shock waves alone or combined with raloxifene promote bone formation and suppress resorption in ovariectomized rats. pubmed:28158228 |
Epilepsy Research | The effect of lamotrigine and phenytoin on bone turnover and bone strength: A prospective study in Wistar rats pubmed:27838501 |
Int Endod J. | Odontoclastogenesis of mouse papilla-derived MDPC-23 cells induced by lipopolysaccharide. pubmed:28333374 |
Sci Rep. | Sprint Interval Training Induces A Sexual Dimorphism but does not Improve Peak Bone Mass in Young and Healthy Mice. pubmed:28303909 |
Sci Rep. | Skeletal Site-specific Effects of Zoledronate on in vivo Bone Remodeling and in vitro BMSCs Osteogenic Activity. pubmed:28139685 |
Biomedicine & Pharmacotherapy | Anti-osteoporotic effects of an antidepressant tianeptine on ovariectomized rats S0753332216322752 |
BMJ Journals | SAT0003 The Role of 12/15-Lipoxygenase in The Pathogenesis of Osteoporosis in Mice content:75 |
Japanese Society for Bone and Mineral Research | Allogeneic yet major histocompatibility complex‑matched bone marrow transplantation in mice results in an impairment of osteoblasts and a significantly reduced trabecular bone article:10.1007 |
ONCOLOGY LETTERS | Assessment of bone metabolism and biomechanical properties of the femur, following treatment with anastrozole and letrozole in an experimental model of menopause ol.2017.6596 |
Journal of Clinical Periodontology | Peroxisome proliferator‐activated receptor γ plays dual roles on experimental periodontitis in rats Pubmed:29574908 |
Journal of Functional Foods | Effect of long-term administration of mangiferin from on bone metabolism in ovariectomized rats 10.1016:j.jff.2018.04.048 |
International Immunopharmacology | Switched memory B cells promote alveolar bone damage during periodontitis: An adoptive transfer experiment Pubmed:30015235 |
European Journal of Pharmacology | The effect of levetiracetam on rat bone mineral density, bone structure and biochemical markers of bone metabolism Pubmed:29428468 |
Journal of Bone and Mineral Metabolism | Allogeneic yet major histocompatibility complex-matched bone marrow transplantation in mice results in an impairment of osteoblasts and a significantly reduced … Pubmed:28766138 |
Experimental and Therapeutic Medicine | Liraglutide exerts a bone‑protective effect in ovariectomized rats with streptozotocin‑induced diabetes by inhibiting osteoclastogenesis Pubmed:29805533 |
Bone | The sole and combined effect of simvastatin and platelet rich fibrin as a filling material in induced bone defect in tibia of albino rats Pubmed: 30208342 |
Journal of Periodontal Research | Improved RANKL expression and osteoclastogenesis induction of CD27+ CD38− memory B cells: A link between B cells and alveolar bone damage in periodontitis Pubmed: 30346027 |
Bone Research | NUMB maintains bone mass by promoting degradation of PTEN and GLI1 via ubiquitination in osteoblasts Pubmed: 30455992 |
Journal of International Dental and Medical Research | Alendronate Effect in RANKL Concentration at Ovariectomized Sprague Dawley Rat. |
Bone | Development and characterization of supramolecular calcitonin assembly and assessment of its interactions with the bone remodelling process Pubmed: 30797058 |
Journal of Oral and Maxillofacial Surgery, Medicine, and Pathology | Evaluation of therapeutic effects of teriparatide in a rat model of zoledronic acid-induced bisphosphonate-related osteonecrosis |
Rheumatology International | Comparison of RANKL expression, inflammatory markers, and cardiovascular risk in patients with acute coronary syndrome with and without rheumatoid arthritis Pubmed: 31297563 |
World Journal of Pediatrics | Regulation of JAK/STAT signal pathway by miR-21 in the pathogenesis of juvenile idiopathic arthritis Pubmed: 31641939 |
Bone | Mice lacking plastin-3 display a specific defect of cortical bone acquisition Pubmed: 31678489 |
BIOSCIENCE REPORTS | IL-38 restrains inflammatory response of collagen-induced arthritis in rats via SIRT1/HIF-1α signaling pathway Pubmed: 32347300 |
Journal of Ethnopharmacology | Anti-osteoporosis effect of Semen Cuscutae in ovariectomized mice through inhibition of bone resorption by osteoclasts 34801609 |
Br J Haematol | Inducible T‐cell co‐stimulator (ICOS) and ICOS ligand are novel players in the multiple‐myeloma microenvironment Pubmed:34954822 |
Biomed Pharmacother | Jiangu granule ameliorated OVX rats bone loss by modulating gut microbiota-SCFAs-Treg/Th17 axis Pubmed:35453007 |
Phytomedicine | Transcriptomic profiling revealed the role of apigenin-4′-O-α-L-rhamnoside in inhibiting the activation of rheumatoid arthritis fibroblast-like synoviocytes via MAPK … Pubmed:35660352 |
Med Science | Identification of marker proteins in preterm and term birth: Relationship of rankl and HSP70 in the cord blood |