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Active C Reactive Protein (CRP)

C-RP; PTX1; Pentraxin-Related; Pentraxin 1

ACTIVITY TEST

Reactive protein (CRP) is an annular (ring-shaped), pentameric protein found in blood plasma, whose levels rise in response to inflammation. It is an acute-phase protein of hepatic origin that increases following interleukin-6 secretion by macrophages and T cells. Its physiological role is to bind to lysophosphatidylcholine expressed on the surface of dead or dying cells (and some types of bacteria) in order to activate the complement system via C1q. Besides, Coagulation Factor II (F2) has been identified as an interactor of CRP, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant rabit CRP and recombinant rat F2. Briefly, CRP was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to F2-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti-CRP pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37℃, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 µL stop solution to the wells and read at 450/630 nm immediately. The binding activity of recombinant rabbit CRP and recombinant rat F2 was shown in Figure 1, the EC50 for this effect is 0.02 ug/mL.

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Experimental Animals Anti-Inflammatory Effect of Guan-Xin-Er-Hao via the Nuclear Factor-Kappa B Signaling Pathway in Rats with Acute Myocardial Infarction Jstage: source
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BMC Neurology Relationships among serum C-reactive protein, receptor for advanced glycation products, metabolic dysfunction, and cognitive impairments PubMed: PMC3765839
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Scientific Report Among overweight middle-aged men, first-borns have lower insulin sensitivity than second-borns Pubmed: 24503677
International Urology Nephrology Perirenal fat associated with microalbuminuria in obese rats Pubmed: 24526332
AJBM Effect of caffeic acid on doxorubicin induced cardiotoxicity in rats Ajbm: Source
Journal of Molecular and Cellular Cardiology Effect of high free fatty acids on the anti-contractile response of perivascular adipose tissue in rat aorta Pubmed: 23939490
PLoS One. Flos Lonicera Ameliorates Obesity and Associated Endotoxemia in Rats through Modulation of Gut Permeability and Intestinal Microbiota Plos: Source
Fundamental & Clinical Pharmacology The determination of histopathological and biochemical effects of the rabbit knee joint injected dexketoprofen trometamol Onlinelibrary: fcp.12074
Mainz Identifizierung neuer potentieller Biomarker für das Kolonkarzinom sowie dessen Vorstufen Info:Source
Int J Clin Exp Med. Celastrol may have an anti-atherosclerosis effect in a rabbit experimental carotid atherosclerosis model Pubmed:Pmc4132129
International Urology and Nephrology As If Biomarker Discovery Isn't Hard Enough: The Consequences of Poorly Characterized Reagents Pubmed:24305687
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天津中医药大学学报 巨噬细胞及其相关因子在家兔不稳定斑块中的作用及其机制 Paper:Source
Mediators of Inflammation Effects of Intravenous Injection of PubMed: 26063970
Am J Physiol Heart Circ Physiol Irisin improves endothelial function in obese mice through the AMPK-eNOS pathway PubMed: 26371167
Cell Physiol Biochem Induction of Haemeoxygenase-1 Directly Improves Endothelial Function in Isolated Aortas from Obese Rats through the Ampk-Pi3k/Akt-Enos Pathway PubMed: 26160485
Exp Physiol Renal protective effects of induction of haem oxygenase-1 combined with increased adiponectin on the glomerular vascular endothelial growth factor–nitric oxide axis in obese rats PubMed: 25959017
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Archives of Physiology and Biochemistry Activation of cardiac renin–angiotensin system and plasminogen activator inhibitor-1 gene expressions in oral contraceptive-induced cardiometabolic disorder Pubmed:26934364
Journal of Functional Foods Orange beverage ameliorates high-fat-diet-induced metabolic disorder in mice article:S1756464616300688
Medical Science Monitor Variation of Circulating Inflammatory Mediators in Staphylococcus aureus and Escherichia coli BloodstreamInfection. Pubmed:26772168
J Diabetes Res.  Central Administration of Galanin Receptor 1 Agonist Boosted Insulin Sensitivity in Adipose Cells of Diabetic Rats pmc:PMC4835658
Asian J Pharm Clin Res THE ANTI-HYPERCHOLESTEROLEMIC EFFECT OF ULVAN POLYSACCHARIDE EXTRACTED FROM THE GREEN ALGA ULVAFASCIATA ON AGED HYPERCHOLESTEROLEMIC RATS journals:10835
J Bone Miner Res Fat and Sucrose Intake Induces Obesity‐Related Bone Metabolism Disturbances: Kinetic and Reversibility Studies in Growing and Adult Rats Pubmed:26175082
Naunyn-Schmiedeberg's Archives of Pharmacology Combined oral contraceptive-induced hypertension is accompanied by endothelial dysfunction and upregulated intrarenal angiotensin II type 1 receptor gene expression pubmed:27447455
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Journal of Diabetes Research Central administration of galanin receptor 1 agonist boosted insulin sensitivity in adipose pubmed:27127795
Therapeutics and Clinical Risk Management Elevated blood plasma levels of epinephrine, norepinephrine, tyrosine hydroxylase, TGFβ1, and TNFα associated with high-altitude pulmonary edema in an Indian population. pubmed:27540296
Veterinary World Acute phase response in lame crossbred dairy cattle pubmed:27956769
J Ethnopharmacol Anti-atherosclerosis and cardio-protective effects of the Angong Niuhuang Pill on a high fat and vitamin D3 induced rodent model of atherosclerosis. pubmed:27880884
SHOCK INTERLEUKIN-1 RECEPTOR 2: A NEW BIOMARKER FOR SEPSIS DIAGNOSIS AND GRAM-NEGATIVE/GRAM-POSITIVE BACTERIAL DIFFERENTIATION pubmed:27984536
International Surgery PROCALCITONIN, C-REACTIVE PROTEIN AND FIBRINOGEN LEVELS IN CASES WITH MECHANIC BOWEL OBSTRUCTION AND THEIR CHANGE IN RELATION TO THE DURATION OF OBSTRUCTION: AN EXPERIMENTAL STUDY 10.3892/ol.2017.7301
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Oncotarget The immune impact of mimic endoscopic retrograde appendicitis therapy and appendectomy on rabbits of acute appendicitis. pubmed:29029533
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Korean Journal of Internal Medicine The relationship between anti-C-reactive protein and disease activity in patients with systemic lupus erythematosus. pubmed:28352058
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Pharmacological reports Anti-inflammatory and antithrombotic effects of nicotine exposure in oral contraceptive-induced insulin resistance are glucocorticoid-independent pubmed:28349880
Nature Communications A ligand-specific blockade of the integrin Mac-1 selectively targets pathologic inflammation while maintaining protective host-defense Pubmed:29410422
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Journal of Experimental Animal Science  Oxymatrine attenuates lipopolysaccharide-induced acute lung injury by activating the epithelial sodium channel and suppressing the JNK signaling pathway Pubmed:29526865
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Journal of Pharmacological Sciences Improved endogenous epoxyeicosatrienoic acid production mends heart function via increased PGC 1α-mitochondrial functions in metabolic syndrome Pubmed: 30342783
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МАРКЕРЫ КОСТНОГО РЕМОДЕЛИРОВАНИЯ ПРИ ЗАМЕЩЕНИИ ДЕФЕКТАТРАБЕКУЛЯРНОЙ КОСТНОЙ ТКАНИ РЕЗОРБИРУЕМЫМИ И …
J Transl Med Multi-omic signatures of atherogenic dyslipidaemia: pre-clinical target identification and validation in humans 33407555
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Sci Rep Ileal transposition helps to regulate plasma hepatokine levels in obese Zucker (Crl: ZUC (ORL)-Lepr fa) rats 33833309
Mol Med Rep Baicalin relieves Mycoplasma pneumoniae infection?induced lung injury through regulating microRNA?221 to inhibit the TLR4/NF?¦ÊB signaling pathway 34109422
Nutrients Prevalence and Factors Associated with Iron Deficiency and Anemia among Residents of Urban Areas of S?o Paulo, Brazil 34072813
peerj Perfluorooctane sulfonate exerts inflammatory bowel disease-like intestinal injury in rats 33510972
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