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Active Haptoglobin (Hpt)

HP; Hp2-Alpha; Alpha-2-Macroglobulin; Zonulin

ACTIVITY TEST

Haptoglobin (Hpt) is an acute-phase plasma glycoprotein primarily produced by hepatocytes. It serves crucial roles in iron homeostasis and antioxidant defense by binding free hemoglobin (Hb) released during hemolysis, forming an irreversible Hpt-Hb complex. This complex is rapidly cleared via macrophage CD163 receptors, preventing Hb-induced oxidative tissue damage and iron loss. As a sensitive acute-phase reactant, Hpt levels elevate markedly during inflammation, infection, or tissue injury, making it a valuable clinical marker. Notably, Hpt can also bind myoglobin (MYO) - though with lower affinity than Hb - potentially alleviating MYO-mediated oxidative stress in rhabdomyolysis or muscle injury.Thus a functional binding ELISA assay was conducted to detect the interaction of recombinant rat Hpt and recombinant bovine MYO . Briefly, biotin-linked Hpt were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100μl were then transferred to MYO-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST 3 times and incubation with Streptavidin-HRP for 30min, then wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µl stop solution to the wells and read at 450nm immediately. The binding activity of recombinant rat Hpt and recombinant bovine MYO was shown in Figure 1, the EC50 for this effect is 0.57 µg/mL.

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Catalog No. Related products for research use of Rattus norvegicus (Rat) Organism species Applications (RESEARCH USE ONLY!)
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RPA817Ra01 Recombinant Haptoglobin (Hpt) Positive Control; Immunogen; SDS-PAGE; WB.
RPA817Ra03 Recombinant Haptoglobin (Hpt) Positive Control; Immunogen; SDS-PAGE; WB.
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PAA817Ra02 Polyclonal Antibody to Haptoglobin (Hpt) WB; IHC
LAA817Ra71 Biotin-Linked Polyclonal Antibody to Haptoglobin (Hpt) WB
MAA817Ra22 Monoclonal Antibody to Haptoglobin (Hpt) WB
MAA817Ra21 Monoclonal Antibody to Haptoglobin (Hpt) WB
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