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Active Lactoferrin (LTF)

HLF2; LF; GIG12; Lactotransferrin; Growth-inhibiting protein 12; Talalactoferrin; Lfcin-H; Kaliocin-1

ACTIVITY TEST

Lactotransferrin (LTF), also known as actoferrin (LF) , is a multifunctional protein of the transferrin family. Lactoferrin belongs to the innate immune system. Apart from its main biological function, namely binding and transport of iron ions, lactoferrin also has antibacterial, antiviral, antiparasitic, catalytic, anti-cancer, and anti-allergic functions and properties. LTF is widely represented in various secretory fluids, such as milk, saliva, tears, and nasal secretions. It also present in secondary granules of PMN and is secreted by some acinar cells. Besides, Ceruloplasmin(CP) has been identified as an interactor of LTF, thus a binding ELISA assay was conducted to detect the interaction of recombinant pig LTF and recombinant pig CP. Briefly, LTF were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100ul were then transferred to CP-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-LTF pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution , wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of LTF and CP was shown in Figure 1, and this effect was in a dose dependent manner.

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
The International Journal of Biochemistry & Cell Biology Changes of hepatic lactoferrin gene expression in two mouse models of the acute phase reaction ScienceDirect: S1357272511002524
BMC Veterinary Research Changes in composition of colostrum of Egyptian buffaloes and Holstein cows BioMed: 17466148
Plos One p38MAPK, ERK and PI3K Signaling Pathways Are Involved in C5a-Primed Neutrophils for ANCA-Mediated Activation PlosOne: source
The Journal of Biological Chemistry Sphingolipid Pathway Regulates Innate Immune Responses at the Fetomaternal Interface during Pregnancy Pubmed:25505239
PLoS One Control of Methicillin-Resistant Staphylococcus aureus Pneumonia Utilizing TLR2 Agonist Pam3CSK4 Pubmed:26974438
Bioscience, Biotechnology, and Biochemistry Salivary lactoferrin is transferred into the brain via the sublingual route pubmed:28351211
Pulmonary Pharmacology & Therapeutics Effects of fluticasone propionate and budesonide on the expression of immune defense genes in bronchial epithelial cells Pubmed:29627483
Microbial Pathogenesis TLR2 agonist Pam3CSK4 enhances the antibacterial functions of GM-CSF induced neutrophils to methicillin-resistant Staphylococcus aureus
Assessment of the Diagnostic Value of IL-10 and Lactoferrin by BIRADS Categories in Breast Neoplasms
J Clin Med A Three-Protein Panel to Support the Diagnosis of Sepsis in Children Pubmed:35329889
Food Funct Three Urban of China: A Cohort Study of Maternal-Infant Factors and HM Protein Components Pubmed:35438093
Biomedicines Myeloid-Specific Pyruvate-Kinase-Type-M2-Deficient Mice Are Resistant to Acute Lung Injury Pubmed:35625931
Catalog No. Related products for research use of Sus scrofa; Porcine (Pig) Organism species Applications (RESEARCH USE ONLY!)
RPA780Po01 Recombinant Lactoferrin (LTF) Positive Control; Immunogen; SDS-PAGE; WB.
APA780Po01 Active Lactoferrin (LTF) Cell culture; Activity Assays.
PAA780Po01 Polyclonal Antibody to Lactoferrin (LTF) WB; IHC; ICC; IP.
LAA780Po81 FITC-Linked Polyclonal Antibody to Lactoferrin (LTF) WB; IHC; ICC; IF.
LAA780Po71 Biotin-Linked Polyclonal Antibody to Lactoferrin (LTF) WB; IHC; ICC.
MAA780Po21 Monoclonal Antibody to Lactoferrin (LTF) WB; IHC; ICC; IP.
SEA780Po ELISA Kit for Lactoferrin (LTF) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA780Po Multiplex Assay Kit for Lactoferrin (LTF) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.