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Active Lactoferrin (LTF)

HLF2; LF; GIG12; Lactotransferrin; Growth-inhibiting protein 12; Talalactoferrin; Lfcin-H; Kaliocin-1

ACTIVITY TEST

Lactoferrin (LF), also known as lactotransferrin (LTF), is a multifunctional protein of the transferrin family. Lactoferrin is one of the components of the immune system of the body; it has antimicrobial activity (bacteriocide, fungicide) and is part of the innate defense, mainly at mucoses. In particular, lactoferrin provides antibacterial activity to human infants. Lactoferrin interacts with DNA and RNA, polysaccharides and heparin, and shows some of its biological functions in complexes with these ligands. LTF is known to activate immune cells to produce several cytokines, such as tumor necrosis factor TNF-a, thus, a stimulation assay was conducted to detect the activity of LTF using human monocytic cell line THP-1. Briefly, THP-1 cells were seeded into wells of 24-well plates at a density of 5×106 cells/mL in RPMI-1640 with the addition of various concentrations of recombinant human LTF. After incubation for 20 hours, the concentration of TNF-a in the cell supernatant was detected using an ELISA kit. TNF-a levels in the cell supernatant of spleen cells increased significantly after stimulated with LTF, the data was shown in table 1.

Figure. TNF-a concentration in the cell supernatant of THP-1 cells up-regulated by LTF.

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
The International Journal of Biochemistry & Cell Biology Changes of hepatic lactoferrin gene expression in two mouse models of the acute phase reaction ScienceDirect: S1357272511002524
BMC Veterinary Research Changes in composition of colostrum of Egyptian buffaloes and Holstein cows BioMed: 17466148
Plos One p38MAPK, ERK and PI3K Signaling Pathways Are Involved in C5a-Primed Neutrophils for ANCA-Mediated Activation PlosOne: source
The Journal of Biological Chemistry Sphingolipid Pathway Regulates Innate Immune Responses at the Fetomaternal Interface during Pregnancy Pubmed:25505239
PLoS One Control of Methicillin-Resistant Staphylococcus aureus Pneumonia Utilizing TLR2 Agonist Pam3CSK4 Pubmed:26974438
Bioscience, Biotechnology, and Biochemistry Salivary lactoferrin is transferred into the brain via the sublingual route pubmed:28351211
Pulmonary Pharmacology & Therapeutics Effects of fluticasone propionate and budesonide on the expression of immune defense genes in bronchial epithelial cells Pubmed:29627483
Microbial Pathogenesis TLR2 agonist Pam3CSK4 enhances the antibacterial functions of GM-CSF induced neutrophils to methicillin-resistant Staphylococcus aureus
Assessment of the Diagnostic Value of IL-10 and Lactoferrin by BIRADS Categories in Breast Neoplasms
J Clin Med A Three-Protein Panel to Support the Diagnosis of Sepsis in Children Pubmed:35329889
Food Funct Three Urban of China: A Cohort Study of Maternal-Infant Factors and HM Protein Components Pubmed:35438093
Biomedicines Myeloid-Specific Pyruvate-Kinase-Type-M2-Deficient Mice Are Resistant to Acute Lung Injury Pubmed:35625931
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
NPA780Hu01 Native Lactoferrin (LTF) Positive Control; Immunogen; SDS-PAGE; WB.
APA780Hu01 Active Lactoferrin (LTF) Cell culture; Activity Assays.
RPA780Hu01 Recombinant Lactoferrin (LTF) Positive Control; Immunogen; SDS-PAGE; WB.
RPA780Hu02 Recombinant Lactoferrin (LTF) Positive Control; Immunogen; SDS-PAGE; WB.
PAA780Hu01 Polyclonal Antibody to Lactoferrin (LTF) WB; IHC; ICC; IP.
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PAA780Hu05 Polyclonal Antibody to Lactoferrin (LTF) WB; IHC; ICC; IP.
LAA780Hu81 FITC-Linked Polyclonal Antibody to Lactoferrin (LTF) WB; IHC; ICC; IF.
LAA780Hu71 Biotin-Linked Polyclonal Antibody to Lactoferrin (LTF) WB; IHC; ICC.
MAA780Hu22 Monoclonal Antibody to Lactoferrin (LTF) WB; IHC; ICC; IP.
LAA780Hu72 Biotin-Linked Monoclonal Antibody to Lactoferrin (LTF) WB; IHC; ICC.
LAA780Hu82 FITC-Linked Monoclonal Antibody to Lactoferrin (LTF) WB; IHC; ICC; IF.
SEA780Hu ELISA Kit for Lactoferrin (LTF) Enzyme-linked immunosorbent assay for Antigen Detection.
SCA780Hu CLIA Kit for Lactoferrin (LTF) Chemiluminescent immunoassay for Antigen Detection.
LMA780Hu Multiplex Assay Kit for Lactoferrin (LTF) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
KSA780Hu01 ELISA Kit DIY Materials for Lactoferrin (LTF) Main materials for "Do It (ELISA Kit) Yourself".