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Active A Disintegrin And Metalloprotease 10 (ADAM10)
CD156-C; CD156c; MADM; Kuz; CDw156; Kuzbanian protein homolog; Mammalian disintegrin-metalloprotease
- Product No.APA766Hu01
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Buffer FormulationPBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
- Traits Freeze-dried powder
- Purity> 90%
- Isoelectric Point6.5
- ApplicationsCell culture; Activity Assays.
- DownloadInstruction Manual
- UOM 10µg50µg 200µg 1mg 5mg
- FOB
US$ 259
US$ 648
US$ 1296
US$ 3888
US$ 9720
For more details, please contact local distributors!
ACTIVITY TEST
A Disintegrin and Metalloprotease 10 (ADAM10) is a membrane-anchored enzyme belonging to the ADAM family. It functions as a sheddase, cleaving the extracellular domains of numerous transmembrane proteins. This activity regulates key biological processes including cell adhesion, signaling, and proteolysis. ADAM10 is essential for embryonic development and is involved in the cleavage of substrates such as Notch, amyloid precursor protein (APP), and E-cadherin. Its function is critical for neural development, immune response, and tissue regeneration.Besides,A Disintegrin And Metalloprotease 17 (ADAM17) has been identified as an interactor of ADAM10, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human ADAM10 and recombinant rat ADAM17. Briefly, ADAM10 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to ADAM17-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti-ADAM10 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37℃, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 µL stop solution to the wells and read at 450/630nm immediately. The binding activity of recombinant human ADAM10 and recombinant rat ADAM17 was shown in Figure 1, the EC50 for this effect is 0.136 µg/mL.
USAGE
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
STORAGE
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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| Magazine | Citations |
| Journal of Allergy and Clinical Immunology | RAGE processing in chronic airway conditions: Involvement of Staphylococcus aureus and ECP ScienceDirect: S0091674912003508 |
| British journal of cancer | ADAM10: a new player in breast cancer progression? PubMed: 26284334 |
| Int Wound J. | From varices to venous ulceration: the story of chronic venous disease described by metalloproteinases. Pubmed:26991748 |
| International Wound Journal | From varices to venous ulceration: the story of chronic venous disease described by metalloproteinases pubmed:26991748 |
| Disease Markers | Adamalysines as Biomarkers and a Potential Target of Therapy in Colorectal Cancer Patients: Preliminary Results Pubmed: 31565100 |
| ADAM10 Plasma Levels Predict Worsening in Cognition of Older Adults: A 3-Year Follow-Up Study | |
| Alzheimers Research & Therapy | ADAM10 plasma levels predict worsening in cognition of older adults: a 3-year follow-up study. 33419480 |
| life | High Levels of TNF-¦Á and TIM-3 as a Biomarker of Immune Reconstitution Inflammatory Syndrome in People with HIV Infection |