≡

Active Fibroblast Growth Factor 23 (FGF23)

ADHR; HYPF; HPDR2; PHPTC; Phosphatonin; Tumor-derived hypophosphatemia-inducing factor

ACTIVITY TEST

Fibroblast growth factor 23 (FGF23) is a 3032 kDa member of the FGF family, within a subfamily that also includes FGF19 and FGF21. FGF23 is produced by osteocytes and osteoblasts in response to high circulating phosphate levels, elevated parathyroid hormone, and circulatory volume loading and it plays a central role in chronic kidney disease-mineral bone disorder and is associated with CKD progression and cardiovascular morbidity. Researches show that FGF23 can directly targets cardiac myocytes via Fibroblast Growth Factor Receptor 4 (FGFR4), thereby inducing hypertrophic myocyte growth and the development of left ventricular hypertrophy (LVH) in rodents. Thus a functional binding ELISA assay was conducted to detect the interaction of recombinant mouse FGF23 and recombinant human FGFR4. Briefly, biotin-linked FGF23 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to FGFR4-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST 3 times and incubation with Streptavidin-HRP for 30min, then wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 µl stop solution to the wells and read at 450 nm immediately. The binding activity of FGF23 and FGFR4 was shown in Figure 1, the EC50 for this effect is 0.074 ug/mL.

USAGE

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
Journal of international medicine Prognostic impact of renal function in precapillary pulmonary hypertension Pubmed: 24011362
BMC Nephrology FGF-23 associated with the progression of coronary artery calcification in hemodialysis patients Pubmed: 24180481
Journal of Clinical Densitometry Lower Fibroblast Growth Factor 23 Levels in Young Adults With Crohn Disease as a Possible Secondary Compensatory Effect on the Disturbance of Bone and Mineral Metabolism Pubmed: 23623649
J Intern Med. Prognostic impact of renal function in precapillary pulmonary hypertension. Pubmed:24011362
Journal of Bone and Mineral Research Adverse effects of osteocytic constitutive activation of ?-catenin on bone strength and bone growth Pubmed:25639729
Nefrologia Fibroblast growth factor is associated to left ventricular mass index, anemia and low values of transferrin saturation El factor de crecimiento fibroblástico está asociado con el índice de masa ventricular izquierda, anemia y niveles bajos de saturación de la transferrina PubMed: 26394828
Medicine (Baltimore) An Attempt to Evaluate Selected Aspects of “Bone–Fat Axis” Function in Healthy Individuals and Patients With Pancreatic Cancer PubMed: 26266370
Ann Biol Clin (Paris) Les biomarqueurs des calcifications vasculaires: quelles limites analytiques pour leur transfert de la recherche bioclinique à la pratique? PubMed: 26069069
Scandinavian Journal of Gastroenterology High-dose fast infusion of parenteral iron isomaltoside is efficacious in inflammatory bowel disease patients with iron-deficiency anaemia without profound changes in phosphate or fibroblast growth factor 23 Pubmed:27326766
PLOS ONE Prostaglandin-E2 Mediated Increase in Calcium and Phosphate Excretion in a Mouse Model ofDistal Nephron Salt Wasting. pubmed:27442254
Journal of Laboratory Diagnostics Evaluation of concentration fibroblast growth factor FGF-23 in hemodialysed patients and after kidney transplantation publication:312592751
Journal of Integrative Nephrology & Andrology Associations between serum fibroblast growth factor 23 level and intradialytic hypotension in hemodialysis patients issn:2394-2916
Journal of Renal Nutrition ADAM17, a New Player in the Pathogenesis of Chronic Kidney Disease-Mineral and Bone Disorder. pubmed:29056164
Experimental Biology and Medicine(Maywood) Effect of cross-linked chitosan iron (III) on vascular calcification in uremic rats Pubmed:29763365
Psychiatry Research Cerebrospinal fluid FGF23 levels correlate with a measure of impulsivity Pubmed:29677623
BMC Pediatrics Serum and urine FGF23 and IGFBP-7 for the prediction of acute kidney injury in critically ill children Pubmed:29907141
Medical Science Monitor Effect of Salt Intervention on Serum Levels of Fibroblast Growth Factor 23 (FGF23) in Chinese Adults: An Intervention Study Pubmed:29608553
International Journal of Chronic Obstructive Pulmonary Disease Disconnection of pulmonary and systemic arterial stiffness in COPD Pubmed:29881265
Saudi journal of kidney diseases and transplantation Prevalence of cardiac arrhythmia and risk factors in chronic kidney disease patients Pubmed:29970732
Nephrology Dialysis Transplantation High-serum phosphate and parathyroid hormone distinctly regulate bone loss and vascular calcification in experimental chronic kidney disease Pubmed: 30189026
BioFactors  Alteration in serum concentrations of FGF19, FGF21, and FGF23 in patients with urothelial carcinoma Pubmed: 30334297
Biological Trace Element Research Altered Mineral Metabolism and Disequilibrium Between Calcification Promoters and Inhibitors in Chronic Hemodialysis Patients Pubmed: 30847765
Clin Implant Dent Relat Res. Evaluation of FGF‐23 and 25(OH)D3 levels in peri‐implant sulcus fluid in peri‐implant health and diseases Pubmed: 31407857
JACC-Cardiovascular Imaging PREVALANCE AND PROGRESSION OF CARDIOVASCULAR CALCIFICATIONS IN HAEMODIALYSIS PATIENTS Pubmed: 28797410
Saudi J Kidney Dis Transpl Serum fibroblast growth factor 23 levels do not correlate with carotid intima-media thickness in patients with chronic kidney disease Pubmed: 31696838
Biomedicine & Pharmacotherapy MiRNA-192-5p attenuates airway remodeling and autophagy in asthma by targeting MMP-16 and ATG7 Pubmed: 31918268
Is Serum FGF-23 Associated with Subclinic Atherosclerosis in Patients with AA Amyloidosis?
THE RELATIONSHIP BETWEEN ARTERIAL STIFNESS AND 25-(OH) VITAMIN D, FGF 23 IN MAINTENANCE HEMODIALYSIS PATIENTS
THE RELATIONSHIP BETWEEN ARTERIAL STIFFNESS AND 25-(OH) VITAMIN D, FGF 23 IN MAINTENANCE HEMODIALYSIS PATIENTS KRON?K ¡­
Calcif Tissue Int The Measurement and Interpretation of Fibroblast Growth Factor 23 (FGF23) Concentrations Pubmed:35665817
European Journal of Medical Genetics Genetic and clinical profile of patients with hypophosphatemic rickets Pubmed:35738466
African Health Sciences Fibroblast Growth Factor 23 (FGF 23) and intact parathyroid hormone (iPTH) as markers of mineral bone disease among Nigerians with non-diabetic kidney disease
Catalog No. Related products for research use of Mus musculus (Mouse) Organism species Applications (RESEARCH USE ONLY!)
RPA746Mu01 Recombinant Fibroblast Growth Factor 23 (FGF23) Positive Control; Immunogen; SDS-PAGE; WB.
RPA746Mu03 Recombinant Fibroblast Growth Factor 23 (FGF23) Positive Control; Immunogen; SDS-PAGE; WB.
APA746Mu03 Active Fibroblast Growth Factor 23 (FGF23) Cell culture; Activity Assays.
RPA746Mu02 Recombinant Fibroblast Growth Factor 23 (FGF23) Positive Control; Immunogen; SDS-PAGE; WB.
APA746Mu01 Active Fibroblast Growth Factor 23 (FGF23) Cell culture; Activity Assays.
PAA746Mu02 Polyclonal Antibody to Fibroblast Growth Factor 23 (FGF23) WB; IHC; ICC; IP.
PAA746Mu01 Polyclonal Antibody to Fibroblast Growth Factor 23 (FGF23) WB; IHC; ICC; IP.
MEA746Mu Mini Samples ELISA Kit for Fibroblast Growth Factor 23 (FGF23) Enzyme-linked immunosorbent assay for Antigen Detection.
SEA746Mu ELISA Kit for Fibroblast Growth Factor 23 (FGF23) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA746Mu Multiplex Assay Kit for Fibroblast Growth Factor 23 (FGF23) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
KSA746Mu01 ELISA Kit DIY Materials for Fibroblast Growth Factor 23 (FGF23) Main materials for "Do It (ELISA Kit) Yourself".