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Active Toll Like Receptor 2 (TLR2)
CD282; TIL4; Toll/interleukin-1 receptor-like protein 4
- Product No.APA663Hu04
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Buffer FormulationPBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
- Traits Freeze-dried powder
- Purity> 90%
- Isoelectric Point4.6
- ApplicationsCell culture; Activity Assays.
- DownloadInstruction Manual
- UOM 10µg50µg 200µg 1mg 5mg
- FOB
US$ 160
US$ 400
US$ 800
US$ 2400
US$ 6000
For more details, please contact local distributors!
ACTIVITY TEST
TLR2 is a member of TLR family which is type I transmembrane proteins with a large number of extracellular leucine-rich repeats (LRRs) and a cytoplasmic Toll/IL-1 receptor (TIR) domain. Human TLR2 is synthesized as a 784 amino acid precursor that contains a signal sequence (aa 1-18), an extracellular domain (aa 19-588) with approximately 20 LRRs, a transmembrane segment (aa 589-609), and a cytoplasmic TIR domain (aa 610-784). The receptor is expressed on a number of cell types including monocytes, dendritic cells, neutrophils, B cells, endothelial cells and hepatocytes. TLR2 stimulation induces mTORC1 activation through TIRAP, which is essential for TLR2-mediated IFN-γ production. Thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human TLR2 and recombinant human TIRAP. Briefly, TLR2 were diluted serially in PBS, with 0.01% BSA (pH7.4). Duplicate samples of 100 ul were then transferred to TIRAP-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti-TLR2 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 µL stop solution to the wells and read at 450 nm immediately. The binding activity of TLR2 and TIRAP was shown in Figure 1, and this effect was in a dose dependent manner, the EC50 was 0.013 ug/ml.
USAGE
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
STORAGE
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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| Magazine | Citations |
| Chinese Medical Journal | Expression of soluble Toll-like receptors in pleural effusions CMJ: 2010823483999506297 |
| PLoS One | Baicalein Reduces Airway Injury in Allergen and IL-13 Induced Airway Inflammation PubMed: PMC3639905 |
| Clinical and Developmental Immunology | TLR2 in Pleural Fluid Is Modulated by Talc Particles during Pleurodesis PubMed: PMC3529880 |
| Advances in Virology | The Possible Role of TLR2 in Chronic Hepatitis B Patients with Precore Mutation Pubmed: 24187552 |
| J Matern Fetal Neonatal Med. | Cervical and vaginal fluid soluble Toll-like receptor 2 in pregnancies complicated by preterm prelabor rupture of membranes Pubmed:25028801 |
| Respiratory Virology and Immunogenicity Advances in Experimental Medicine and Biology | Cytokines and Toll-Like Receptors in the Immune Response to Influenza Vaccination Pubmed:25248345 |
| Iran J Immunol. | Correlation?between?salivary?Toll like receptor-2?concentration?and early childhood caries. Pubmed:25265998 |
| Int J Dermatol. | Membrane and soluble Toll‐like receptor 2 in patients with psoriasis treated by Goeckerman therapy Pubmed:25266302 |
| BMC Immunology | The discriminative capacity of soluble Toll-like receptor (sTLR)2 and sTLR4 in inflammatory diseases Pubmed:25406630 |
| Pathol Biol (Paris) | Cathelicidin related antimicrobial peptide, laminin, Toll-like receptors and chemokines levels in experimental hypersensitivity pneumonitis in mice PubMed: 25834936 |
| Plos One | Soluble Toll-Like Receptors 2 and 4 in Cerebrospinal Fluid of Patients with Acute Hydrocephalus followingAneurysmal Subarachnoid Haemorrhage. Pubmed:27223696 |
| Open Forum Infect Dis | The Effect of Rosuvastatin on Markers of Immune Activation in Treatment-Naive Human Immunodeficiency Virus-Patients Pubmed:26835476 |
| Perfusion | Pentraxin 3 and other inflammatory biomarkers related to atrial fibrillation in cardiac surgery pubmed:27852958 |
| Journal For Neurovirology | Soluble TLR2 and 4 concentrations in cerebrospinal fluid in HIV/SIV-relatedneuropathological conditions. pubmed:27882497 |
| Acta Orthopaedica | Toll-like receptors and their soluble forms differ in the knee and thumb basal osteoarthritic joints pubmed:28093922 |
| Biological Markers and Guided Therapy | Features of the Pattern Recognition Receptor TLR-2 Expression and Cytokines IL-2, IL-4 Profile in Children with Food Hypersensitivity 10.12988:bmgt.2018.812 |
| CHILD`S HEALTH | Prospective study on the significance of mucosal barrier dysfunction markers in assessing the effectiveness of the new strategy for treating food hypersensitivity in … 10.22141:2224-0551.13.2.2018.129548 |
| Neurological Sciences | Vinpocetine regulates levels of circulating TLRs in Parkinson's disease patients Pubmed: 30315378 |
| Biological Markers in Fundamental and Clinical Medicine | PROGNOSTIC ROLE OF IL-4, IL-2 AND TLR-2 FOR PREDICTING OF COURSE OF FOOD ALLERGY IN CHILDREN Doi: 10.29256/v.02.02.2018.escbm71 |
| Biological Markers in Fundamental and Clinical Medicine | STUDY OF THE ACTION OF CHROMIUM-CONTAINING BIOPREPARATIONS FROM SPIRULINA ON INSULIN ACTIVITY IN RATS Doi: 10.29256/v.02.02.2018.escbm72 |
| Biomedicine & Pharmacotherapy | Opuntioside, opuntiol and its metallic nanoparticles attenuate adjuvant-induced arthritis: Novel suppressors of Toll-like receptors-2 and-4 |
| PLoS One | Aberrant DNA methylation of the toll-like receptors 2 and 6 genes in patients with obstructive sleep apnea Pubmed: 32069296 |
| Georgian medical news | ASSOCIATIONS BETWEEN EFFICACY OF THE THERAPY AND CIRCADIAN FLUCTUATIONS OF ENDOTHELIAL NITRIC OXIDE SYNTHASE WITH TOLL-LIKE … Pubmed: 32242853 |
| Medical and Clinical Chemistry | ДОбОвА вАРІАбЕЛЬНІСТЬ РІвНЯ РОзЧИННИХ ТОЛ-пОДІбНИХ РЕцЕпТОРІв 2 в СИРОвАТцІ КРОвІ ЖІНОК, ХвОРИХ НА РЕвМАТОїДНИй АРТРИТ: зв' … |
| Sciences of Europe | FEATURES OF MYOCARDIAL REMODELING IN MEN WITH POST-INFARCTION CARDIOSCLEROSIS AND ITS RELATIONSHIP WITH TOLL-LIKE … |
| CLINICAL INVESTIGATIONS | Levels of toll-like receptors 2 and 4 in serum of men with postinfarction cardiosclerosis and its relationship with antiphospholipid syndrome components |
| Acta Obstet Gynecol Scand | A value of soluble Toll‐like receptor 2 and 4 in vaginally obtained amniotic fluid for the prediction of histological chorioamnionitis 34244993 |
| Clinical and Translational Allergy | Altered innate immune profile in blood of systemic mastocytosis patients Pubmed:35734269 |