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Active Fibroblast Growth Factor 7 (FGF7)

KGF; HBGF7; Keratinocyte Growth Factor; Heparin-binding growth factor 7

ACTIVITY TEST

Fibroblast Growth Factor 7 (FGF7), also known as keratinocyte growth factor (KGF), is a paracrine signaling protein. It is mainly secreted by mesenchymal cells and specifically acts on epithelial cells. FGF7 plays crucial roles in cell proliferation, migration, and differentiation during development, tissue repair, and regeneration.FGF7 binds to Fibroblast Growth Factor Receptor 2 (FGFR2), particularly the IIIb isoform. This binding initiates a series of signaling cascades that regulate cellular functions essential for tissue homeostasis.Thus a functional ELISA assay was conducted to detect the interaction of recombinant human FGF7 and recombinant rat FGFR2. Briefly, FGF7 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to FGFR2-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti-FGF7 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37℃, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 µL stop solution to the wells and read at 450/630nm immediately. The binding activity of recombinant human FGF7 and recombinant rat FGFR2 was shown in Figure 1, the EC50 for this effect is 0.050ug/mL.

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
Journal of Controlled Release A DPP-IV-resistant glucagon-like peptide-2 dimer with enhanced activity against radiation-induced intestinal injury pubmed:28522195
Inflammation Involvements of γδT Lymphocytes in Acute and Chronic Skin Wound Repair. pubmed:28540539
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
RPA636Hu01 Recombinant Fibroblast Growth Factor 7 (FGF7) Positive Control; Immunogen; SDS-PAGE; WB.
APA636Hu01 Active Fibroblast Growth Factor 7 (FGF7) Cell culture; Activity Assays.
RPA636Hu02 Recombinant Fibroblast Growth Factor 7 (FGF7) Positive Control; Immunogen; SDS-PAGE; WB.
PAA636Hu01 Polyclonal Antibody to Fibroblast Growth Factor 7 (FGF7) WB; IHC; ICC; IP.
MAA636Hu22 Monoclonal Antibody to Fibroblast Growth Factor 7 (FGF7) WB; IHC; ICC; IP.
SEA636Hu ELISA Kit for Fibroblast Growth Factor 7 (FGF7) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA636Hu Multiplex Assay Kit for Fibroblast Growth Factor 7 (FGF7) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.