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Active Granzyme A (GZMA)

CTLA3; HFSP; HF; H factor; Granzyme 1; Fragmentin-1; Hanukkah factor; Cytotoxic T-Lymphocyte-Associated Serine Esterase 3; CTL tryptase; Cytotoxic T-lymphocyte proteinase 1

ACTIVITY TEST

Granzyme A is a member of the granzyme family of the serine proteases found specifically in the cytotoxic granules of cytotoxic T lymphocytes (CTL) and natural killer (NK) cells. Granzyme A is the most abundant protease in CTL and NK cells. It induces caspaseindependent cell death when introduced into target cells by perforin (1). Mouse granzyme A is synthesized as a precursor (262 residues) with a signal peptide (residues 126), a propeptide (residues 27-28) and a mature chain (residues 29-262). The purified recombinant mouse Granzyme A consists of residues 29 to 262 which activity was measured by its ability to cleaves a thioester substrate Z-Lys-SBzl•HCl. The reaction was performed in 0.05 M Tris, 0.15 M NaCl, 0.01% Triton X-100, pH 8.0 (Assay Buffer), initiated by addition 50 μL of various concentrations of GZMA (dilute by Assay Buffer) to 50 µL of 1.2 mM Substrate and DTNB mixture. The final well serves as a negative control with no GZMA, replace with 50μL assay buffer. Incubated at 25℃ for 5min, then read at a wavelength of 405 nm. The specific activity of recombinant mouse Granzyme A is 6214 pmol/min/µg.

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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INCREMENT SERVICES

Magazine Citations
Cell Reports Granzyme A Produces Bioactive IL-1β through a Nonapoptotic Inflammasome-Independent Pathway Pubmed:25437548
Journal of Dermatological Science Relationships among plasma granzyme B level, pruritus and dermatitis in patients with atopicdermatitis. pubmed:27686401
Journal of Maternal-Fetal & Neonatal Medicine Extracellular granzyme A in amniotic fluid is elevated in the presence of sterile intra-amniotic inflammation in preterm prelabor rupture of membranes Pubmed: 32912008
Catalog No. Related products for research use of Mus musculus (Mouse) Organism species Applications (RESEARCH USE ONLY!)
RPA599Mu01 Recombinant Granzyme A (GZMA) Positive Control; Immunogen; SDS-PAGE; WB.
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EPA599Mu61 Eukaryotic Granzyme A (GZMA) Positive Control; Immunogen; SDS-PAGE; WB.
APA599Mu61 Active Granzyme A (GZMA) Cell culture; Activity Assays.
PAA599Mu02 Polyclonal Antibody to Granzyme A (GZMA) WB; IHC; ICC; IP.
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LAA599Mu71 Biotin-Linked Polyclonal Antibody to Granzyme A (GZMA) WB; IHC; ICC.
SEA599Mu ELISA Kit for Granzyme A (GZMA) Enzyme-linked immunosorbent assay for Antigen Detection.
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KSA599Mu01 ELISA Kit DIY Materials for Granzyme A (GZMA) Main materials for "Do It(ELISA Kit) Yourself"