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Active Myoglobin (MYO)

PVALB; MB

ACTIVITY TEST

Myoglobin (symbol Mb or MB or MYO) is an iron- and oxygen-binding protein found in the muscle tissue of vertebrates in general and in almost all mammals. It is related to hemoglobin, which is the iron- and oxygen-binding protein in blood, specifically in the red blood cells. Myoglobin is found in Type I muscle, Type II A and Type II B, but most texts consider myoglobin not to be found in smooth muscle. Besides, Proteasome 26S Subunit, Non ATPase 4 (PSMD4) has been identified as an interactor of MYO, thus a binding ELISA assay was conducted to detect the interaction of recombinant human MYO and recombinant human PSMD4 Briefly, MYO were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100μL were then transferred to PSMD4-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-MYO pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of MYO and PSMD4 was shown in Figure 1, and this effect was in a dose dependent manner.
Figure. The binding activity of MYO with PSMD4.

USAGE

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
European Journal of Applied Physiology Acute leukocyte, cytokine and adipocytokine responses to maximal and hypertrophic resistance exercise bouts Pubmed:25145982
International Journal of Molecular Sciences Transcriptional Alterations by Ischaemic Postconditioning in a Pig Infarction Model: Impact on Microvascular Protection Pubmed: 30650650
BIOSENS BIOELECTRON Electrochemical dual-aptamer-based biosensor for nonenzymatic detection of cardiac troponin I by nanohybrid electrocatalysts labeling combined with DNA … Pubmed: 30954926
Mikrochim Acta DNA nanotetrahedron linked dual-aptamer based voltammetric aptasensor for cardiac troponin I using a magnetic metal-organic framework as a label Pubmed: 31123904
ACS Appl Mater Interfaces Emitter–Quencher Pair of Single Atomic Co Sites and Monolayer Titanium Carbide MXenes for Luminol Chemiluminescent Reactions 34914377
Talanta A rapid fluorescent aptasensor for point-of-care detection of C-reactive protein Pubmed:35714415
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
RPA480Hu01 Recombinant Myoglobin (MYO) Positive Control; Immunogen; SDS-PAGE; WB.
RPA480Hu02 Recombinant Myoglobin (MYO) Positive Control; Immunogen; SDS-PAGE; WB.
APA480Hu01 Active Myoglobin (MYO) Cell culture; Activity Assays.
PAA480Hu01 Polyclonal Antibody to Myoglobin (MYO) WB; IHC; ICC; IP.
PAA480Hu02 Polyclonal Antibody to Myoglobin (MYO) WB; IHC; ICC; IP.
LAA480Hu81 FITC-Linked Polyclonal Antibody to Myoglobin (MYO) WB; IHC; ICC; IF.
LAA480Hu71 Biotin-Linked Polyclonal Antibody to Myoglobin (MYO) WB; IHC; ICC.
MAA480Hu26 Monoclonal Antibody to Myoglobin (MYO) WB; IHC; ICC; IP.
MAA480Hu28 Monoclonal Antibody to Myoglobin (MYO) WB; IHC; ICC; IP.
MAA480Hu23 Monoclonal Antibody to Myoglobin (MYO) WB; IHC; ICC; IP.
MAA480Hu21 Monoclonal Antibody to Myoglobin (MYO) WB; IHC; ICC; IP.
MAA480Hu25 Monoclonal Antibody to Myoglobin (MYO) WB; IHC; ICC; IP.
MAA480Hu24 Monoclonal Antibody to Myoglobin (MYO) WB; IHC; ICC; IP.
MAA480Hu27 Monoclonal Antibody to Myoglobin (MYO) WB; IHC; ICC; IP.
MAA480Hu29 Monoclonal Antibody to Myoglobin (MYO) WB; IHC; ICC; IP.
MAA480Hu22 Monoclonal Antibody to Myoglobin (MYO) WB; IHC; ICC; IP.
FAA480Hu04 Anti-Myoglobin (MYO) Monoclonal Antibody Flow cytometry.
FAA480Hu02 Anti-Myoglobin (MYO) Monoclonal Antibody Flow cytometry.
SEA480Hu ELISA Kit for Myoglobin (MYO) Enzyme-linked immunosorbent assay for Antigen Detection.
SCA480Hu CLIA Kit for Myoglobin (MYO) Chemiluminescent immunoassay for Antigen Detection.
LMA480Hu Multiplex Assay Kit for Myoglobin (MYO) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
KSA480Hu01 ELISA Kit DIY Materials for Myoglobin (MYO) Main materials for "Do It (ELISA Kit) Yourself".