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Active Mineralocorticoid Receptor (MR)

NR3C2; MLR; MCR; Aldosterone Receptor; Nuclear Receptor Subfamily 3 Group C Member 2

ACTIVITY TEST

The Mineralocorticoid Receptor (MR) is a ligand-activated transcription factor that mediates aldosterone signaling, regulating electrolyte balance and blood pressure in the kidney. Beyond its classical role, MR influences inflammation, fibrosis, and cardiovascular pathophysiology. As a member of the nuclear receptor superfamily, MR requires chaperone proteins for proper folding and function. Heat Shock Protein 90kDa Alpha B1 (HSP90aB1) is a molecular chaperone that stabilizes MR in its inactive state, facilitating hormone binding and nuclear translocation upon activation. Thus a functional ELISA assay was conducted to detect the interaction of recombinant human MR and recombinant human HSP90aB1. Briefly, MR was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to HSP90aB1-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti-MR pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37℃, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 µL stop solution to the wells and read at 450/630nm immediately. The binding activity of recombinant human MR and recombinant human HSP90aB1 was shown in Figure 1, the EC50 for this effect is 1.68 μg/mL.

USAGE

Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
Journal of Food Science  Effects of Hyriopsis cumingii Polysaccharides on Mice Immunologic Receptor, Transcription Factor, and Cytokine Pubmed:27061742
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
APA464Hu01 Active Mineralocorticoid Receptor (MR) Cell culture; Activity Assays.
RPA464Hu01 Recombinant Mineralocorticoid Receptor (MR) Positive Control; Immunogen; SDS-PAGE; WB.
PAA464Hu01 Polyclonal Antibody to Mineralocorticoid Receptor (MR) WB; IHC
MAA464Hu22 Monoclonal Antibody to Mineralocorticoid Receptor (MR) WB; IHC; ICC; IP.
SEA464Hu ELISA Kit for Mineralocorticoid Receptor (MR) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA464Hu Multiplex Assay Kit for Mineralocorticoid Receptor (MR) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.