Active Galectin 8 (GAL8)
LGALS8; PCTA1; Po66-CBP; Prostate Carcinoma Tumor Antigen 1; Lectin,Galactoside-Binding Soluble 8; Po66 carbohydrate-binding protein; Prostate carcinoma tumor antigen 1
- Product No.APA308Hu01
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Buffer FormulationPBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
- Traits Freeze-dried powder
- Purity> 95%
- Isoelectric Point8.3
- ApplicationsCell culture; Activity Assays.
- DownloadInstruction Manual
- UOM 10µg50µg 200µg 1mg 5mg
- FOB
US$ 302
For more details, please contact local distributors! US$ 756 US$ 1512 US$ 4536 US$ 11340
ACTIVITY TEST
Galectin 8 (GAL8), also known as prostate carcinoma tumor antigen 1 (PCTA1) in human, is a tandem repeat-type galectin. is a member of the lectin family, of which 14 mammalian galectins have been identified. It is also a member of the beta-galactoside-binding protein family that plays an important role in cell-cell adhesion, cell-matrix interactions, macrophage activation, angiogenesis, metastasis, apoptosis. In this case, we chose rabbit erythrocyte (RaE) to assay its ability of agglutination. A general procedure for hemagglutination assay (or haemagglutination assay; HA) is as follows, two-fold dilute the recombinant human GAL8 with 0.9% sodium chloride injection, add 50μL a serial dilution of GAL8 to each well of a U or V-bottom shaped 96-well microtiter plate. The final well serves as a negative control with no GAL8, replace with 50μL 0.9% sodium chloride injection. Then add 50μL 1% rabbit erythrocyte to each well and mixed gently. The plate is incubated for 3 hours at room temperature. The results are shown in Figure 1. It was obvious that the minimal effective concentration of GAL8 is 6.25μg/mL.
Figure 2. The hemagglutination assay of GAL8 in V- bottom shaped 96-well microtiter plate.
USAGE
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
STORAGE
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
GIVEAWAYS
INCREMENT SERVICES
- BCA Protein Quantification Kit
- Molecular Mass Marker for Protein
- Monoclonal Antibody Customized Service
- Polyclonal Antibody Customized Service
- Protein Activity Test Experiment Service
- Electrophoretic Mobility Shift Assay (EMSA) Experiment Service
- Buffer
- Lentivirus Packaging Experiment Service
- Adenovirus Packaging Experiment Service
- Real Time PCR Experimental Service
- Spike RBD Protein (S-RBD)
- Protein G
- Protein A
Magazine | Citations |
Cell Metabolism | Podoplanin-Expressing Macrophages Promote Lymphangiogenesis and Lymphoinvasion in Breast Cancer Pubmed: 31447322 |
CALCIFIED TISSUE INTERNATIONAL | The Dysregulated Galectin Network Activates NF-κB to Induce Disease Markers and Matrix Degeneration in 3D Pellet Cultures of Osteoarthritic Chondrocytes Pubmed: 33185768 |
Catalog No. | Related products for research use of Homo sapiens (Human) Organism species | Applications (RESEARCH USE ONLY!) |
RPA308Hu01 | Recombinant Galectin 8 (GAL8) | Positive Control; Immunogen; SDS-PAGE; WB. |
APA308Hu01 | Active Galectin 8 (GAL8) | Cell culture; Activity Assays. |
PAA308Hu01 | Polyclonal Antibody to Galectin 8 (GAL8) | WB; IHC; ICC; IP. |
LAA308Hu71 | Biotin-Linked Polyclonal Antibody to Galectin 8 (GAL8) | WB; IHC; ICC. |
LAA308Hu81 | FITC-Linked Polyclonal Antibody to Galectin 8 (GAL8) | WB; IHC; ICC; IF. |
MAA308Hu27 | Monoclonal Antibody to Galectin 8 (GAL8) | WB; IHC; ICC; IP. |
MAA308Hu25 | Monoclonal Antibody to Galectin 8 (GAL8) | WB; IHC; ICC; IP. |
MAA308Hu24 | Monoclonal Antibody to Galectin 8 (GAL8) | WB; ICC/IF |
MAA308Hu29 | Monoclonal Antibody to Galectin 8 (GAL8) | WB; IHC; ICC; IP. |
MAA308Hu23 | Monoclonal Antibody to Galectin 8 (GAL8) | WB; IHC; ICC; IP. |
MAA308Hu21 | Monoclonal Antibody to Galectin 8 (GAL8) | WB; IHC; ICC; IP. |
MAA308Hu22 | Monoclonal Antibody to Galectin 8 (GAL8) | WB; IHC; ICC; IP. |
SEA308Hu | ELISA Kit for Galectin 8 (GAL8) | Enzyme-linked immunosorbent assay for Antigen Detection. |
LMA308Hu | Multiplex Assay Kit for Galectin 8 (GAL8) ,etc. by FLIA (Flow Luminescence Immunoassay) | FLIA Kit for Antigen Detection. |
KSA308Hu01 | ELISA Kit DIY Materials for Galectin 8 (GAL8) | Main materials for "Do It (ELISA Kit) Yourself". |